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dc.contributor.authorGarcía Hevia, Lorena 
dc.contributor.authorValiente Barroso, Rafael 
dc.contributor.authorMartín Rodríguez, Rosa 
dc.contributor.authorRenero Lecuna, Carlos 
dc.contributor.authorGonzález Gómez, Jesús Antonio 
dc.contributor.authorRodríguez-Fernández, Lidia
dc.contributor.authorAguado Menéndez, Fernando 
dc.contributor.authorVillegas Sordo, Juan Carlos 
dc.contributor.authorLópez Fanarraga, Mónica 
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2016-05-03T17:09:53Z
dc.date.available2016-05-03T17:09:53Z
dc.date.issued2016-04-25
dc.identifier.issn2040-3364
dc.identifier.issn2040-3372
dc.identifier.otherPI13/01074, AES 2013es_ES
dc.identifier.otherMAT2012-38664-C02-01es_ES
dc.identifier.urihttp://hdl.handle.net/10902/8309
dc.description.abstractZinc is a crucial element in biology that plays chief catalytic, structural and protein regulatory roles. Excess cytoplasmic Zinc is toxic to the cells so there are cell-entry and intracellular buffering mechanisms that control intracellular Zinc disponibility. Tubulin and actin are two Zinc-scavenging proteins that are essential components of the cellular cytoskeleton implicated in cell division, migration and cellular architecture maintenance. Here we demonstrate how exposure to different ZnO nanostructures, namely ZnO commercial nanoparticles and custom-made ZnO nanowires, produce acute cytotoxic effects in human keratinocytes (HaCat) and epithelial cells (HeLa) triggering a dose-dependent cell retraction and collapse. We show how engulfed ZnO nanoparticles dissolve intracellularly, triggering actin filament bundling and structural changes in microtubules, transforming these highly dynamic 24nm diameter polymers into rigid macrotubes of tubulin, severely affecting cell proliferation and survival. Our results demostrate that nano-ZnO causes an acute cytoskeletal collapse that triggers necrosis, followed by a late reactive oxygen species (ROS)-dependent apoptotic process.es_ES
dc.description.sponsorshipThis work has been supported by the Spanish ISCIII-MINECO under Projects ref. PI13/01074, AES 2013; FONDOS FEDER; MAT2012-38664-C02-01. We especially thank the IDIVAL for their support to LGH and the IDIVAL-Microscopy Unit for all the microscopy imaging.es_ES
dc.format.extent23 p.es_ES
dc.language.isoenges_ES
dc.publisherRoyal Society of Chemistryes_ES
dc.rights© Royal Society of Chemistryes_ES
dc.sourceNanoscale. 2016 Jun 7;8(21):10963-73es_ES
dc.titleNano-ZnO leads to tubulin macrotube assembly and actin bundling triggering cytoskeletal catastrophe and cell necrosises_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.1039/C6NR00391E
dc.type.versionsubmittedVersiones_ES


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