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dc.contributor.authorFernández López, Raúl
dc.contributor.authorRuiz González, Raúl
dc.contributor.authorCruz Calahorra, Fernando de la 
dc.contributor.authorMoncalián Montes, Gabriel 
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2015-09-08T06:50:23Z
dc.date.available2015-09-08T06:50:23Z
dc.date.issued2015-07-01
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10902/7098
dc.description.abstractWhole cell biosensors (WCBs) have multiple applications for environmental monitoring, detecting a wide range of pollutants. WCBs depend critically on the sensitivity and specificity of the transcription factor (TF) used to detect the analyte. We describe the mechanism of regulation and the structural and biochemical properties of TF families that are used, or could be used, for the development of environmental WCBs. Focusing on the chemical nature of the analyte, we review TFs that respond to aromatic compounds (XylS-AraC, XylR-NtrC, and LysR), metal ions (MerR, ArsR, DtxR, Fur, and NikR) or antibiotics (TetR and MarR). Analyzing the structural domains involved in DNA recognition, we highlight the similitudes in the DNA binding domains (DBDs) of these TF families. Opposite to DBDs, the wide range of analytes detected by TFs results in a diversity of structures at the effector binding domain. The modular architecture of TFs opens the possibility of engineering TFs with hybrid DNA and effector specificities. Yet, the lack of a crisp correlation between structural domains and specific functions makes this a challenging task.es_ES
dc.format.extent21 p.es_ES
dc.language.isoenges_ES
dc.publisherFrontierses_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.sourceFrontiers in Microbiology. 2015 Jul 1;6:648es_ES
dc.subject.otherAnalytees_ES
dc.subject.otherAromatic compoundses_ES
dc.subject.otherBiosensorses_ES
dc.subject.otherEffectores_ES
dc.subject.otherMetales_ES
dc.subject.otherTranscription factores_ES
dc.titleTranscription factor-based biosensors enlightened by the analytees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.3389/fmicb.2015.00648
dc.type.versionpublishedVersiones_ES


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Atribución 3.0 EspañaExcept where otherwise noted, this item's license is described as Atribución 3.0 España