Measurement of the optical properties of biological media: using dual-integrating spheres and inverse adding-doubling approximation
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AuthorSarrallé Argüelles, Ramón
Nowadays a set of optical devices are used in the biomedical field with therapeutic, surgery and diagnostic purposes. Since early 90's there are already methods available to measure the optical properties we need to characterize biological media. But most of them are complex, expensive and require a lot of time (spectroscopy, interferometry...). Any material can be characterized, in optical terms, if we know the values of their coefficients of absorption [mi]a and scattering [mi]s. Knowing the optical properties of a tissue is extremely important, and also the first step to secure a right and effective photon-tissue interaction. Because from this coefficient values we can get the therapeutic window, that will allow as to set the right values (for: the wavelength, the power, the exposure time...) depending on the purposes we want to achieve (diagnose a maligned tumor, use ablation to perform optical surgery, active some chemical reaction with a certain energy level...). Our goal, was to use a combinative method that mix integrating spheres (IS), Monte Carlo stochastic model (MC) and Inverse adding-doubling approximation (IAD), to obtain [mi]a and [mi]s, and checked if the coefficient values we get are close enough with the values that are already known. So that in future researches we can simply use this IS/MC/IAD method to characterize other biological tissues and uses the coefficients obtained as reliable values.