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dc.contributor.authorCorreia, Susana
dc.contributor.authorNunes Miranda, Júlio D.
dc.contributor.authorPinto, Luís
dc.contributor.authorSantos, Hugo M.
dc.contributor.authorToro Hernando, María de
dc.contributor.authorSáenz Domínguez, Yolanda
dc.contributor.authorTorres Manrique, Carmen
dc.contributor.authorCapelo, José Luis
dc.contributor.authorPoeta, Patrícia
dc.contributor.authorIgrejas, Gilberto
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.description.abstractSalmonellosis is one of the most common and widely distributed foodborne diseases. The emergence of Salmonella strains that are resistant to a variety of antimicrobials is a serious global public health concern. Salmonella enterica serovar Typhimurium definitive phage type 104 (DT104) is one of these emerging epidemic multidrug resistant strains. Here we collate information from the diverse and comprehensive range of experiments on Salmonella proteomes that have been published. We then present a new study of the proteome of the quinolone-resistant Se20 strain (phage type DT104B), recovered after ciprofloxacin treatment and compared it to the proteome of reference strain SL1344. A total of 186 and 219 protein spots were recovered from Se20 and SL1344 protein extracts, respectively, after two-dimensional gel electrophoresis. The signatures of 94% of the protein spots were successfully identified through matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS). Three antimicrobial resistance related proteins, whose genes were previously detected by polymerase chain reaction (PCR), were identified in the clinical strain. The presence of these proteins, dihydropteroate synthase type-2 (sul2 gene), aminoglycoside resistance protein A (strA gene) and aminoglycoside 6'-N-acetyltransferase type Ib-cr4 (aac(6')-Ib-cr4 gene), was confirmed in the DT104B clinical strain. The aac(6')-Ib-cr4 gene is responsible for plasmid-mediated aminoglycoside and quinolone resistance. This is a preliminary analysis of the proteome of these two S. Typhimurium strains and further work is being developed to better understand how antimicrobial resistance is developing in this pathogen.es_ES
dc.format.extent29 p.es_ES
dc.rightsAtribución 3.0 Españaes_ES
dc.sourceInternational Journal of Molecular Sciences. 2014 Aug 15;15(8):14191-219es_ES
dc.titleComplete proteome of a quinolone-resistant Salmonella Typhimurium phage type DT104B clinical straines_ES

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Atribución 3.0 EspañaExcept where otherwise noted, this item's license is described as Atribución 3.0 España