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dc.contributor.authorCaraballo Otero, Juan Manuel
dc.contributor.authorAcosta Cobacho, Juan Carlos
dc.contributor.authorCortés Vázquez, Miguel Ángel
dc.contributor.authorAlbajar Molera, Marta
dc.contributor.authorGómez Casares, María Teresa
dc.contributor.authorBatllé López, Ana 
dc.contributor.authorCuadrado, María Ángeles
dc.contributor.authorOnaindia Pérez, Arantza
dc.contributor.authorBretones Sánchez, Gabriel
dc.contributor.authorLlorca Díaz, Francisco Javier 
dc.contributor.authorPiris Pinilla, Miguel Ángel 
dc.contributor.authorColomer Pujol, Dolors
dc.contributor.authorLeón Serrano, Javier 
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.description.abstractMyc (c-Myc) counteracts p27 effects, and low p27 usually correlates with high Myc expression in human cancer. However there is no information on the co-expression of both genes in chronic lymphocytic leukemia (CLL). We found a lack of correlation between RNA and protein levels of p27 and Myc in CLL cells, so we determined the protein levels by immunoblot in 107 cases of CLL. We observed a high p27 protein expression in CLL compared to normal B cells. Ectopic p27 expression in a CLL-derived cell line resulted in cell death resistance. Surprisingly, Myc expression was very low or undetectable in most CLL cases analyzed, with a clear correlation between high p27 and low Myc protein levels. This was associated with low Skp2 expression, which is consistent with the Skp2 role in p27 degradation and with SKP2 being a Myc target gene. High Myc expression did not correlate with leukemia progression, despite that cell cycle-related Myc target genes were upregulated. However, biochemical analysis showed that the high p27 levels inhibited cyclin-Cdk complexes even in Myc expressing CLL cells. Our data suggest that the combination of high p27 and low Myc is a marker of CLL cells which is mediated by Skp2.es_ES
dc.format.extent15 p.es_ES
dc.publisherImpact Journalses_ES
dc.rightsAtribución 3.0 Españaes_ES
dc.sourceOncotarget. 2014 Jul 15;5(13):4694-708es_ES
dc.titleHigh p27 protein levels in chronic lymphocytic leukemia are associated to low Myc and Skp2 expression, confer resistance to apoptosis and antagonize Myc effects on cell cyclees_ES

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Atribución 3.0 EspañaExcept where otherwise noted, this item's license is described as Atribución 3.0 España