Show simple item record

dc.contributor.authorAlperi Vega, Anabel
dc.contributor.authorLarrea, Delfina
dc.contributor.authorFernández González, Esther
dc.contributor.authorDehio, Christoph
dc.contributor.authorZechner, Ellen L.
dc.contributor.authorLlosa Blas, Matxalen 
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2013-12-16T12:24:51Z
dc.date.available2013-12-16T12:24:51Z
dc.date.issued2013-08-30
dc.identifier.issn0021-9193
dc.identifier.urihttp://hdl.handle.net/10902/4049
dc.description.abstractType IV secretion system (T4SS) substrates are recruited through a translocation signal that is poorly defined for conjugative relaxases. The relaxase TrwC of plasmid R388 is translocated by its cognate conjugative T4SS, and it can also be translocated by the VirB/D4 T4SS of Bartonella henselae, causing DNA transfer to human cells. In this work, we constructed a series of TrwC variants and assayed them for DNA transfer to bacteria and human cells to compare recruitment requirements by both T4SSs. Comparison with other reported relaxase translocation signals allowed us to determine two putative translocation sequence (TS) motifs, TS1 and TS2. Mutations affecting TS1 drastically affected conjugation frequencies, while mutations affecting either motif had only a mild effect on DNA transfer rates through the VirB/D4 T4SS of B. henselae. These results indicate that a single substrate can be recruited by two different T4SSs through different signals. The C terminus affected DNA transfer rates through both T4SSs tested, but no specific sequence requirement was detected. The addition of a Bartonella intracellular delivery (BID) domain, the translocation signal for the Bartonella VirB/D4 T4SS, increased DNA transfer up to 4% of infected human cells, providing an excellent tool for DNA delivery to specific cell types. We show that the R388 coupling protein TrwB is also required for this high-efficiency TrwC-BID translocation. Other elements apart from the coupling protein may also be involved in substrate recognition by T4SSs.es_ES
dc.format.extent8 p.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.rights© American Society for Microbiologyes_ES
dc.sourceJournal of Bacteriology. 2013 Nov;195(22):4999-5006. Epub 2013 Aug 30.es_ES
dc.titleA translocation motif in relaxase TrwC specifically affects recruitment by its conjugative type IV secretion systemes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.1128/JB.00367-13
dc.type.versionpublishedVersiones_ES


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record