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dc.contributor.authorDíaz Mendoza, Manuel Jesús 
dc.contributor.authorLorda Diez, Carlos Ignacio 
dc.contributor.authorMontero Simón, Juan Antonio 
dc.contributor.authorGarcía-Porrero Pérez, Juan Antonio 
dc.contributor.authorHurlé González, Juan Mario 
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.description.abstractInterdigital cell death is a physiological regression process responsible for sculpturing the digits in the embryonic vertebrate limb. Changes in the intensity of this degenerative process account for the different patterns of interdigital webbing among vertebrate species. Here, we show that Reelin is present in the extracellular matrix of the interdigital mesoderm of chick and mouse embryos during the developmental stages of digit formation. Reelin is a large extracellular glycoprotein which has important functions in the developing nervous system, including neuronal survival; however, the significance of Reelin in other systems has received very little attention. We show that reelin expression becomes intensely downregulated in both the chick and mouse interdigits preceding the establishment of the areas of interdigital cell death. Furthermore, fibroblast growth factors, which are cell survival signals for the interdigital mesoderm, intensely upregulated reelin expression, while BMPs, which are proapototic signals, downregulate its expression in the interdigit. Gene silencing experiments of reelin gene or its intracellular effector Dab-1 confirmed the implication of Reelin signaling as a survival factor for the limb undifferentiated mesoderm. We found that Reelin activates canonical survival pathways in the limb mesoderm involving protein kinase B and focal adhesion kinase. Our findings support that Reelin plays a role in interdigital cell death, and suggests that anoikis (apoptosis secondary to loss of cell adhesion) may be involved in this process.es_ES
dc.format.extent7 p.es_ES
dc.publisherNature Publishing Groupes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.sourceCell Death and Disease. 2013 Sep 12;4:e800es_ES
dc.titleInterdigital cell death in the embryonic limb is associated with depletion of Reelin in the extracellular matrixes_ES

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Atribución-NoComercial-SinDerivadas 3.0 EspañaExcept where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España