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    Fluorescence imaging contrast in guided surgery on nerves measured in rats in vivo

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    FluorescenceImagingC ... (279.2Kb)
    Identificadores
    URI: http://hdl.handle.net/10902/21038
    DOI: 10.1117/12.2544665
    ISSN: 0277-786X
    ISSN: 1996-756X
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    Author
    Fanjul Vélez, FélixAutoridad Unican; Díaz Martínez, Álvaro Marcelino; Garro Martínez, Emilio; Arce Diego, José LuisAutoridad Unican
    Date
    2020-02-19
    Derechos
    © 2020 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
    Publicado en
    Proceedings of SPIE, 2020, 11222, 112220K
    Molecular-Guided Surgery: Molecules, Devices, and Applications VI, San Francisco, California (Online), 2020
    Publisher
    SPIE Society of Photo-Optical Instrumentation Engineers
    Enlace a la publicación
    https://doi.org/10.1117/12.2544665
    Palabras clave
    Fluorescence
    Biomarkers
    Nerve tissue
    In vivo animal imaging
    Guided-surgery
    Abstract:
    An appropriate identification of the tissue type that is in front of the excision surgical tool is critical, for either tumoral tissue removal, or even accessory healthy tissues whose destruction could cause severe collateral damage. Healthy tissue distinction is an unsolved task in real surgical praxis, due for instance to alterations or hemorrhages. Many approaches have tried to solve the problem of healthy tissue discrimination, such as spectroscopy. Diffuse Reflectance Spectroscopy (DRS), an easy to implement and reliable optical diagnostic technique, has shown great potential in tumoral tissue discrimination, as long as in healthy tissue distinction. However, the identification of nerve tissue in particular is challenging. What is more, nerve tissue distinction is critical in surgical interventions, due to the severe irreversible dysfunctions in patient mobility that collateral damage could cause. Fluorescence is an optical technique that could be of relevance for nerve identification. The need of specific fluorophores that tend to accumulate preferentially on nerve tissue is essential for this task. Image quality requires also an analysis of the optical source, filters employed, fluorophore concentration or inoculation method. Together with those aspects, additional open questions include incubation time or cytotoxicity, critical for clinical translation. In this work, fluorescence imaging for nerve identification is applied on in vivo rats. The approach uses several fluorophore concentrations, based on Oxazine, and combines topical and intravascular inoculation. Optical irradiance is considered for image contrast. The results show an appreciable nerve contrast for optimized parameters.
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    UNIVERSIDAD DE CANTABRIA

    Repositorio realizado por la Biblioteca Universitaria utilizando DSpace software
    Contact Us | Send Feedback
    Metadatos sujetos a:licencia de Creative Commons Reconocimiento 3.0 España