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dc.contributor.authorGodin, Isabelle
dc.contributor.authorGarcía-Porrero Pérez, Juan Antonio 
dc.contributor.authorDieterlen-Lièvre, Françoise
dc.contributor.authorCumano, Ana
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.description.abstractIn the mouse embryo, the generation of candidate progenitors for long-lasting hemopoiesis has been reported in the paraaortic splanchnopleura (P-Sp)/ aorta-gonad-mesonephros (AGM) region. Here, we address the following question: can the P-Sp/AGM environment support hemopoietic differentiation as well as generate stem cells, and, conversely, are other sites where hemopoietic differentiation occurs capable of generating stem cells? Although P-Sp/AGM generates de novo hemopoietic stem cells between 9.5 and 12.5 days post coitus (dpc), we show here that it does not support hemopoietic differentiation. Among mesoderm-derived sites, spleen and omentum were shown to be colonized by exogenous cells in the same fashion as the fetal liver. Cells colonizing the spleen were multipotent and pursued their evolution to committed progenitors in this organ. In contrast, the omentum, which was colonized by lymphoid-committed progenitors that did not expand, cannot be considered as a hemopoietic organ. From these data, stem cell generation appears incompatible with hemopoietic activity. At the peak of hemopoietic progenitor production in the P-Sp/AGM, between 10.5 and 11.5 dpc, multipotent cells were found at the exceptional frequency of 1 out of 12 total cells and 1 out of 4 AA4.1+ cells. Thus, progenitors within this region constitute a pool of undifferentiated hemopoietic cells readily accessible for characterization.es_ES
dc.format.extent10 p.es_ES
dc.publisherRockefeller University Presses_ES
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Españaes_ES
dc.sourceJournal of Experimental Medicine. 1999 Jul 5;190(1):43-52es_ES
dc.titleStem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic siteses_ES

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Atribución-NoComercial-CompartirIgual 3.0 EspañaExcept where otherwise noted, this item's license is described as Atribución-NoComercial-CompartirIgual 3.0 España