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dc.contributor.authorTorregrosa-Hetland, Cristina J.
dc.contributor.authorVillanueva, José 
dc.contributor.authorGiner, Daniel
dc.contributor.authorLópez-Font, Inmaculada
dc.contributor.authorNadal, Ángel
dc.contributor.authorExpósito-Romero, Giovanna
dc.contributor.authorGil Gómez, Amparo 
dc.contributor.authorGonzález Vélez, Virginia
dc.contributor.authorSegura Sala, José Javier 
dc.contributor.authorGutiérrez Pérez, Luis Miguel
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2013-02-11T14:41:02Z
dc.date.available2013-02-11T14:41:02Z
dc.date.issued2011-03-01
dc.identifier.issn1477-9137
dc.identifier.issn0021-9533
dc.identifier.urihttp://hdl.handle.net/10902/1619
dc.description.abstractWe have studied how the F-actin cytoskeleton is involved in establishing the heterogeneous intracellular Ca2+ levels ([Ca2+]i) and in the organization of the exocytotic machinery in cultured bovine chromaffin cells. Simultaneous confocal visualization of [Ca2+]i and transmitted light studies of the cytoskeleton showed that, following cell stimulation, the maximal signal from the Ca2+-sensitive fluorescent dye Fluo-3 was in the empty cytosolic spaces left by cytoskeletal cages. This was mostly due to the accumulation of the dye in spaces devoid of cytoskeletal components, as shown by the use of alternative Ca2+-insensitive fluorescent cytosolic markers. In addition to affecting the distribution of such compounds in the cytosol, the cytoskeleton influenced the location of L- and P-Q-type Ca2+ channel clusters, which were associated with the borders of cytoskeletal cages in resting and stimulated cells. Indeed, syntaxin-1 and synaptotagmin-1, which are components of the secretory machinery, were present in the same location. Furthermore, granule exocytosis took place at these sites, indicating that the organization of the F-actin cytoskeletal cortex shapes the preferential sites for secretion by associating the secretory machinery with preferential sites for Ca2+ entry. The influence of this cortical organization on the propagation of [Ca2+]i can be modelled, illustrating how it serves to define rapid exocytosis.es_ES
dc.format.extent8 p.es_ES
dc.language.isoenges_ES
dc.publisherThe Company of Biologistes_ES
dc.rights© 2011. Published by The Company of Biologists Ltdes_ES
dc.sourceJournal of Cell Science, 2011, 124 (5), 727-734.es_ES
dc.subject.otherChromaffin celles_ES
dc.subject.otherExocytosises_ES
dc.subject.otherF-actin cytoskeletones_ES
dc.subject.otherIntracellular Ca2+es_ES
dc.subject.otherSNARE proteines_ES
dc.subject.otherFluorescence microscopyes_ES
dc.titleThe F-actin cortical network is a major factor influencing the organization of the secretory machinery in chromaffin cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherVersionhttp://dx.doi.org/10.1242/jcs.078600
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.1242/​jcs.078600
dc.type.versionpublishedVersiones_ES


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