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dc.contributor.authorGonzález González, Alicia
dc.contributor.authorGonzález Cabeza, Alicia Verónica 
dc.contributor.authorAlonso González, Carolina 
dc.contributor.authorMenéndez Menéndez, Javier
dc.contributor.authorMartínez Campa, Carlos Manuel 
dc.contributor.authorCos Corral, Samuel 
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2019-01-22T17:15:57Z
dc.date.available2019-01-22T17:15:57Z
dc.date.issued2018-01
dc.identifier.issn1021-335X
dc.identifier.issn1791-2431
dc.identifier.otherSAF2013-42012-P ; SAF2016-77103-P
dc.identifier.otherSAF2013-42012-Pes_ES
dc.identifier.otherSAF2016-77103-Pes_ES
dc.identifier.urihttp://hdl.handle.net/10902/15470
dc.description.abstractMelatonin exerts oncostatic activity in breast cancer through antiangiogenic actions. There, the aim of the present study was to ascertain whether melatonin modulates, in a coordinated action, angiopoietin-1 (ANG-1), ANG-2, their cognate Tie2 receptor and VEGF in co-cultures of human endothelial cells (HUVECs) and breast cancer (MCF-7) cells. To accomplish this we used co-cultures of human breast cancer cells (MCF-7) or non-malignant human mammary epithelial cells (MCF?10A) with endothelial cells (HUVECs). The presence of breast cancer cells increased HUVEC proliferation and 1 mM melatonin prevented this effect. ANG-1, ANG-2 and VEGF levels in co-culture media and mRNA expression were upregulated and Tie2 mRNA expression was downregulated in the HUVECs and MCF-7. Melatonin (1 mM) downregulated ANG-1, ANG-2 and VEGF levels in the co-culture media and mRNA expression in both types of cells and upregulated Tie2 mRNA expression in HUVECs. ANG-1, ANG-2, Tie2 and VEGF mRNA expression were not modified during HUVEC/MCF-10A co-culture. Estradiol (10 nM) increased ANG-1, ANG-2 and VEGF mRNA expression in HUVECs and melatonin (1 mM) counteracted this effect. We conclude that melatonin simultaneously coordinates downregulation of angiopoietins with a reduction in VEGF, which could be an effective therapeutic strategy for blocking tumor angiogenesis.es_ES
dc.description.sponsorshipThe present study was supported by grants from the Spanish Economy and Competitiveness Ministry (SAF2013-42012-P, SAF2016-77103-P), and from the Instituto de Investigación Sanitaria Valdecilla (IDIVAL) (APG/12).es_ES
dc.format.extent9 p.es_ES
dc.language.isoenges_ES
dc.publisherSpandidos Publicationses_ES
dc.rights© Spandidos Publicationses_ES
dc.sourceOncol Rep. 2018 Jan;39(1):433-441es_ES
dc.subject.otherMelatonines_ES
dc.subject.otherEndothelial Cellses_ES
dc.subject.otherBreast Canceres_ES
dc.subject.otherAngiopoietinses_ES
dc.subject.otherHUVECes_ES
dc.subject.otherVEGFes_ES
dc.titleComplementary actions of melatonin on angiogenic factors,the angiopoietin/Tie2 axis and VEGF, in co-cultures of human endothelial and breast cancer cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherVersionhttps://dx.doi.org/10.3892/or.2017.6070es_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.3892/or.2017.6070
dc.type.versionpublishedVersiones_ES


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