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dc.contributor.authorLi, Hegang
dc.contributor.authorSong, Xiaona
dc.contributor.authorYang, Feng
dc.contributor.authorBao, Hanxun
dc.contributor.authorLu, Xiaolong
dc.contributor.authorPérez Campo, Flor María
dc.contributor.authorZhao, Jinshan
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2019-01-21T15:00:25Z
dc.date.available2019-01-21T15:00:25Z
dc.date.issued2018-01
dc.identifier.issn1791-2997
dc.identifier.issn1791-3004
dc.identifier.urihttp://hdl.handle.net/10902/15438
dc.description.abstractConditional gene targeting at porcine I?B? may be a solution to delayed xenograft rejection, the main barrier to xenotransplantation. An oligonucleotide?based method was applied to construct the vector for conditional targeting of porcine I?B?. This method was free from PCR amplification during the assembling of the different vector elements, avoiding introduction of unwanted mutations. With the help of short double?stranded DNA fragments produced by annealing oligonucleotides, nondirectional cloning has also been avoided. By making the best of directional cloning, a highly complex targeting vector was built within 3 weeks. The present study also explained why the two recombination?based methods (recombineering and gateway recombination), although having demonstrated to be highly efficient in constructing ordinary targeting vectors, were not appropriate in this context. The description in the present study of an additional method to efficiently construct targeting vectors is suggested to introduce more flexibility in the field therefore helping to meet the different needs of the researchers.es_ES
dc.description.sponsorshipThe present study was funded by the National Natural Science Foundation of China (grants no. 31301936 and 31572383) and Project of Qingdao People's Livelihood Science and Technology (grant no. 14 2 3 45 nsh).es_ES
dc.format.extent7 p.es_ES
dc.language.isoenges_ES
dc.publisherSpandidos Publicationses_ES
dc.rights© Spandidos Publicationses_ES
dc.sourceMol Med Rep. 2018 Jan;17(1):653-659es_ES
dc.subject.otherTargeting Vector Constructiones_ES
dc.subject.otherOligonucleotideses_ES
dc.subject.otherXenotransplantationes_ES
dc.subject.otherPorcine IκBαes_ES
dc.titleApplication of oligonucleotides to construct a conditional targeting vector for porcine IKBaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherVersionhttps://dx.doi.org/10.3892/mmr.2017.7917es_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.3892/mmr.2017.7917
dc.type.versionpublishedVersiones_ES


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