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dc.contributor.authorToro, María de
dc.contributor.authorFernández, Javier
dc.contributor.authorGarcía, Vanesa
dc.contributor.authorMora, Azucena
dc.contributor.authorBlanco, Jorge
dc.contributor.authorCruz Calahorra, Fernando de la 
dc.contributor.authorRodicio, M. Rosario
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2018-02-26T19:10:02Z
dc.date.available2018-02-26T19:10:02Z
dc.date.issued2017
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10902/13097
dc.description.abstractCarbapenem-resistant Enterobacteriaceae, including the increasingly reported OXA-48 Escherichia coli producers, are an emerging public health threat worldwide. Due to their alarming detection in our healthcare setting and their possible presence in the community, seven OXA-48-producing, extraintestinal pathogenic E. coli were analysed by whole genome sequencing as well as conventional tools, and tested for in vivo virulence. As a result, five E. coli OXA-48-producing subclones were detected (O25:H4-ST131/PST43-fimH30-virotype E; O25:H4-ST131/PST9-fimH22-virotype D5, O16:H5-ST131/ PST506-fimH41; O25:H5-ST83/PST207 and O9:H25-ST58/PST24). Four ST131 and one ST83 isolates satisfied the ExPEC status, and all except the O16:H5 ST131 isolate were UPEC. All isolates exhibited local inflammatory response with extensive subcutaneous necrosis but low lethality when tested in a mouse sepsis model. The blaOXA-48 gene was located in MOBP131/IncL plasmids (four isolates) or within the chromosome (three ST131 H30-Rx isolates), carried by Tn1999-like elements. All, except the ST83 isolate, were multidrug-resistant, with additional plasmids acting as vehicles for the spread of various resistance genes. This is the first study to analyse the whole genome sequences of blaOXA-48-positive ST131, ST58 and ST83 E. coli isolates in conjunction with experimental data, and to evaluate the in vivo virulence of blaOXA-48 isolates, which pose an important challenge to patient management.es_ES
dc.format.extent12 p.es_ES
dc.language.isoenges_ES
dc.publisherNature Publishing Groupes_ES
dc.rights© The author(s) 2017. Atribución 4.0 Internacionales_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceScientific Reports 2017, 7: 12103es_ES
dc.titleWhole genome sequencing,molecular typing and in vivovirulence of OXA-48-producingEscherichia coli isolates includingST131 H30-Rx, H22 and H41subcloneses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.1038/s41598-017-12015-0
dc.type.versionpublishedVersiones_ES


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© The author(s) 2017. Atribución 4.0 InternacionalExcept where otherwise noted, this item's license is described as © The author(s) 2017. Atribución 4.0 Internacional