Improvement in the definition of anti-HLA antibody profile in highly sensitized patients
EstadísticasView Usage Statistics
Full recordShow full item record
AuthorIrure, Juan; Asensio, Esther; Rodrigo Calabia, Emilio; Romón Alonso, Íñigo; Gómez, Javier; Arias Rodríguez, Manuel; López Hoyos, Marcos; San Segundo Arribas, David
Atribución 3.0 España
PLoS One. 2017; 12(2): e0171463
Public Library of Science
The definition of anti-HLA antibody profile in highly sensitized patients on a waiting list is crucial when virtual crossmatch is used in organ allocation systems, but also when used to identify the true deleterious anti-HLA antibodies. Here we propose different levels of risk based on the results of anti-HLA antibody testing in neat serum (N) and after sera dilution (DIL) and C1q test in 18 highly sensitized patients. This group was heterogeneous in terms of anti-HLA antibody titers and their ability to fix complement. After dilution, 15 out of 18 patients (83.3%) showed a reduction of positive bead counts whereas 4 patients showed a prozone effect and complement fixation was demonstrated. The high dilution of sera and ascertaining the complement fixation allow the accurate definition of risk anti-HLA antibody profiles in highly sensitized patients, as demonstrated in 5 of the sensitized patients who were transplanted.