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dc.contributor.authorGutiérrez, Olga
dc.contributor.authorBerciano Blanco, María Teresa 
dc.contributor.authorLafarga Coscojuelo, Miguel Ángel
dc.contributor.authorFernández Luna, José Luis 
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.description.abstractBACKGROUND: Thyrotroph embryonic factor (TEF), a member of the PAR bZIP family of transcriptional regulators, has been involved in neurotransmitter homeostasis, amino acid metabolism, and regulation of apoptotic proteins. In spite of its relevance, nothing is known about the regulation of TEF. PRINCIPAL FINDINGS: p53-dependent genotoxic agents have been shown to be much more harmful for PAR bZIP-deficient mice as compared to wild type animals. Here we demonstrate that TEF expression is controlled by p53 through upregulation of microRNA-125b, as determined by both regulating the activity of p53 and transfecting cells with microRNA-125b precursors. We also describe a novel role for TEF in controlling actin distribution and cell shape in mouse fibroblasts. Lack of TEF is accompanied by dramatic increase of cell area and decrease of elongation (bipolarity) and dispersion (multipolarity). Staining of actin cytoskeleton also showed that TEF (-/-) cells are characterized by appearance of circumferential actin bundles and disappearance of straight fibers. Interestingly, transfection of TEF (-/-) fibroblasts with TEF induced a wild type-like phenotype. Consistent with our previous findings, transfection of wild type fibroblasts with miR-125b promoted a TEF (-/-)-like phenotype, and a similar but weaker effect was observed following exogenous expression of p53. CONCLUSIONS/SIGNIFICANCE: These findings provide the first evidence of TEF regulation, through a miR-125b-mediated pathway, and describes a novel role of TEF in the maintenance of cell shape in fibroblasts.es_ES
dc.format.extent8 p.es_ES
dc.publisherPublic Library of Sciencees_ES
dc.rightsAtribución 3.0 Españaes_ES
dc.sourcePLoS One. 2011 Feb 11;6(2):e17169es_ES
dc.titleA novel pathway of TEF regulation mediated by microRNA-125b contributes to the control of actin distribution and cell shape in fibroblastses_ES

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Atribución 3.0 EspañaExcept where otherwise noted, this item's license is described as Atribución 3.0 España