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dc.contributor.authorAguirre Quiñonero, A.es_ES
dc.contributor.authorCano García, María Eliezeres_ES
dc.contributor.authorGamal, D.es_ES
dc.contributor.authorCalvo Alén, Jaime es_ES
dc.contributor.authorMartínez Martínez, Luis es_ES
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2017-09-11T07:12:02Z
dc.date.available2018-07-01T02:45:09Z
dc.date.issued2017es_ES
dc.identifier.issn0732-8893es_ES
dc.identifier.issn1879-0070es_ES
dc.identifier.urihttp://hdl.handle.net/10902/11855
dc.description.abstractObjectives The objective of this study was to evaluate the accuracy of the CIM test in the detection of carbapenemase activity in 124 strains of Enterobacteriaceae. Methods A panel of 124 previously characterized Enterobacteriaceae was tested: 77 strains producing the following carbapenemase families: KPC (n = 14), GES (n = 22), NDM (n = 19), VIM (n = 4), IMP (n = 4) and OXA-48 (n = 14) and 47 non-carbapenemase producers. For the CIM method, an active susceptibility meropenem disc was exposed to a bacterial suspension of a test strain; when a carbapenemase is produced, the antibiotic is inactivated allowing uninhibited growth of an indicator strain after overnight incubation. A clear inhibition zone (?20 mm) was considered indicative of no-carbapenemase activity. Results All KPC, NDM, VIM, IMP or OXA-48 producing strains were unequivocally detected with the CIM test. CIM false negative results were obtained with eleven Enterobacter cloacae producing GES-6. Two other E. cloacae not producing carbapenemase (one with SHV-12, one hyperproducing AmpC) were positive by the test. The sensitivity and specificity of the assay compared to those of molecular methods were 85.7% and 95.7%, respectively. Conclusions The CIM method proved to be inexpensive and easy to interpret. It provided less than optimal results in the detection of GES-6 activity.es_ES
dc.format.extent22 p.es_ES
dc.language.isoenges_ES
dc.publisherElsevier Biomedicales_ES
dc.rights© <2017>. This manuscript version is made available under the CC-BY-NC-ND 4.0 licensees_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceDiagnostic Microbiology and Infectious Disease Volume 88, Issue 3, July 2017, Pages 214-218es_ES
dc.subject.otherCarbapenemasees_ES
dc.subject.otherCarbapenem Inactivation Methodes_ES
dc.subject.otherRapid testes_ES
dc.subject.otherGES-6es_ES
dc.titleEvaluation of the carbapenem inactivation method (CIM) for detecting carbapenemase activity in enterobacteriaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherVersionhttps://doi.org/10.1016/j.diagmicrobio.2017.03.009es_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.1016/j.diagmicrobio.2017.03.009es_ES
dc.type.versionacceptedVersiones_ES


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© <2017>. This manuscript version is made available under the CC-BY-NC-ND 4.0 licenseExcept where otherwise noted, this item's license is described as © <2017>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license