TBCCD1, a new centrosomal protein, is required for centrosome and Golgi apparatus positioning
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AuthorGonçalves, João; Nolasco, Sofía; Nascimento, Rute; López Fanarraga, Mónica; Zabala Otaño, Juan Carlos; Soares, Helena
In animal cells the centrosome is actively positioned at the cell center in close association
with the nucleus. The mechanisms responsible for this are not completely understood.
Here we report the first characterization of human TBCCD1, a protein related to tubulin
cofactor C. TBCCD1 localizes at the centrosome and at the spindle midzone, midbody
and basal bodies of primary and motile cilia. Knockdown of TBCCD1 in RPE-1 cells
caused the dissociation of the centrosome from the nucleus and disorganization of the
Golgi apparatus. TBCCD1 depleted cells are larger, less efficient in primary cilia
assembly and their migration is slower in wound-healing assays. However, the
microtubule nucleating activity of the centrosome is not affected by TBCCD1 silencing.
We propose that TBCCD1 is a key regulator of centrosome positioning and consequently
of internal cell organization.
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