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dc.contributor.authorNavas Méndez, Jesús 
dc.contributor.authorFernández Martínez, Marta
dc.contributor.authorSalas Pelayo, Carlos
dc.contributor.authorEliecer Cano, María
dc.contributor.authorMartínez Martínez, Luis 
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2017-05-26T17:37:06Z
dc.date.available2017-05-26T17:37:06Z
dc.date.issued2016
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10902/11097
dc.description.abstractCorynebacterium striatum is an opportunistic pathogen, often multidrug-resistant, which has been associated with serious infections in humans. Aminoglycosides are second-line or complementary antibiotics used for the treatment of Corynebacterium infections. We investigated the susceptibility to six aminoglycosides and the molecular mechanisms involved in aminoglycoside resistance in a collection of 64 Corynebacterium striatum isolated in our laboratory during the period 2005?2009. Antimicrobial susceptibility was determined using E-test. The mechanisms of aminoglycoside resistance were investigated by PCR and sequencing. The 64 C. striatum were assessed for the possibility of clonal spreading by Pulsed-field Gel Electrophoresis (PFGE). Netilmicin and amikacin were active against the 64 C. striatum isolates (MICs90 = 0.38 and 0.5 mg/L, respectively). Twenty-seven of the 64 C. striatum strains showed a MIC90 for kanamycin > 256 mg/L, and 26 out the 27 were positive for the aph(3?)-Ic gene. Thirty-six out of our 64 C. striatum were streptomycin resistant, and 23 out of the 36 carried both the aph(3?)-Ib and aph(6)-Id genes. The gene aac(3)-XI encoding a new aminoglycoside 3-N acetyl transferase from C. striatum was present in 44 out of the 64 isolates, all of them showing MICs of gentamicin and tobramycin > 1 mg/L. CS4933, a C. striatum showing very low susceptibility to kanamycin and streptomycin, contains an aminoglycoside resistance region that includes the aph(3?)-Ic gene, and the tandem of genes aph(3?)-Ib and aph(6)-Id. Forty-six major PFGE types were identified among the 64 C. striatum isolates, indicating that they were mainly not clonal. Our results showed that the 64 clinical C. striatum were highly resistant to aminoglycosides and mostly unrelated.es_ES
dc.format.extent10 p.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.rights© 2016 Navas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.es_ES
dc.sourcePLoS ONE 2016 11(12): e0167856es_ES
dc.titleSusceptibility to Aminoglycosides and Distribution of aph and aac(3)-XI Genes among Corynebacterium striatum Clinical Isolateses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.1371/journal.pone.0167856
dc.type.versionpublishedVersiones_ES


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