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dc.contributor.authorLópez Fanarraga, Mónica 
dc.contributor.authorBellido Sánchez, Javier
dc.contributor.authorJaén, Cristina
dc.contributor.authorVillegas Sordo, Juan Carlos 
dc.contributor.authorZabala Otaño, Juan Carlos 
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2012-11-30T08:56:12Z
dc.date.available2012-11-30T08:56:12Z
dc.date.issued2012-01-22
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10902/1087
dc.description.abstractMicrotubule-organizing centers recruit alpha- and beta-tubulin polypeptides for microtubule nucleation. Tubulin synthesis is complex, requiring five specific cofactors, designated tubulin cofactors (TBCs) A-E, which contribute to various aspects of microtubule dynamics in vivo. Here, we show that tubulin cofactor D (TBCD) is concentrated at the centrosome and midbody, where it participates in centriologenesis, spindle organization, and cell abscission. TBCD exhibits a cell-cycle-specific pattern, localizing on the daughter centriole at G1 and on procentrioles by S, and disappearing from older centrioles at telophase as the protein is recruited to the midbody. Our data show that TBCD overexpression results in microtubule release from the centrosome and G1 arrest, whereas its depletion produces mitotic aberrations and incomplete microtubule retraction at the midbody during cytokinesis. TBCD is recruited to the centriole replication site at the onset of the centrosome duplication cycle. A role in centriologenesis is further supported in differentiating ciliated cells, where TBCD is organized into "centriolar rosettes". These data suggest that TBCD participates in both canonical and de novo centriolar assembly pathways.es_ES
dc.format.extent11 p.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.rightsAtribución 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/
dc.sourcePLoS One. 2010 Jan 22;5(1):e8846.es_ES
dc.titleTBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.1371/journal.pone.0008846
dc.type.versionpublishedVersiones_ES


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Atribución 3.0 EspañaExcept where otherwise noted, this item's license is described as Atribución 3.0 España