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dc.contributor.authorSander, Birgittaes_ES
dc.contributor.authorJong, Daphne dees_ES
dc.contributor.authorRosenwald, Andreases_ES
dc.contributor.authorXie, Wanlinges_ES
dc.contributor.authorBalagué, Olgaes_ES
dc.contributor.authorCalaminici, Mariaes_ES
dc.contributor.authorCarreras, Joaquimes_ES
dc.contributor.authorGaulard, Philippees_ES
dc.contributor.authorGribben, Johnes_ES
dc.contributor.authorHagenbeek, Antones_ES
dc.contributor.authorKersten, Marie Josées_ES
dc.contributor.authorMolina, Thierry Joes_ES
dc.contributor.authorLee, Abigailes_ES
dc.contributor.authorMontes Moreno, Santiago es_ES
dc.contributor.authorOtt, Germanes_ES
dc.contributor.authorRaemaekers, Johnes_ES
dc.contributor.authorSalles, Gilleses_ES
dc.contributor.authorSehn,Lauriees_ES
dc.contributor.authorThorns, Christophes_ES
dc.contributor.authorWahlin, Björn E.es_ES
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2017-03-29T09:58:31Z
dc.date.available2017-03-29T09:58:31Z
dc.date.issued2014es_ES
dc.identifier.issn0390-6078es_ES
dc.identifier.issn1592-8721es_ES
dc.identifier.urihttp://hdl.handle.net/10902/10729
dc.description.abstractThe cellular microenvironment in follicular lymphoma is of biological and clinical importance. Studies on the clinical significance of non-malignant cell populations have generated conflicting results, which may partly be influenced by poor reproducibility in immunohistochemical marker quantification. In this study, the reproducibility of manual scoring and automated microscopy based on a tissue microarray of 25 follicular lymphomas as compared to flow cytometry is evaluated. The agreement between manual scoring and flow cytometry was moderate for CD3, low for CD4, and moderate to high for CD8, with some laboratories scoring closer to the flow cytometry results. Agreement in manual quantification across the 7 laboratories was low to moderate for CD3, CD4, CD8 and FOXP3 frequencies, moderate for CD21, low for MIB1 and CD68, and high for CD10. Manual scoring of the architectural distribution resulted in moderate agreement for CD3, CD4 and CD8, and low agreement for FOXP3 and CD68. Comparing manual scoring to automated microscopy demonstrated that manual scoring increased the variability in the low and high frequency interval with some laboratories showing a better agreement with automated scores. Manual scoring reliably identified rare architectural patterns of T-cell infiltrates. Automated microscopy analyses for T-cell markers by two different instruments were highly reproducible and provided acceptable agreement with flow cytometry. These validation results provide explanations for the heterogeneous findings on the prognostic value of the microenvironment in follicular lymphoma. We recommend a more objective measurement, such as computer-assisted scoring, in future studies of the prognostic impact of microenvironment in follicular lymphoma patients.es_ES
dc.format.extent10 p.es_ES
dc.language.isoenges_ES
dc.publisherFerrata Storti Foundationes_ES
dc.rightsAtribución-NoComercial 3.0 España. © Ferrata Storti Foundation. Sander B. et al. 2014. The reliability of immunohistochemical analysis of the tumor microenvironment in follicular lymphoma: a validation study from the Lunenburg Lymphoma Biomarker Consortium. "Obtained from the Haematologica Journal website http://www.haematologica.org"*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/es/*
dc.sourcehaematologica | 2014; 99(4), p. 715-725es_ES
dc.titleThe reliability of immunohistochemical analysis of the tumor microenvironment in follicular lymphoma: a validation study from the Lunenburg Lymphoma Biomarker Consortiumes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.3324/haematol.2013.095257es_ES
dc.type.versionpublishedVersiones_ES


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Atribución-NoComercial 3.0 España. © Ferrata Storti Foundation. Sander B. et al. 2014. The reliability of immunohistochemical analysis of the tumor microenvironment in follicular lymphoma: a validation study from the Lunenburg Lymphoma Biomarker Consortium. "Obtained from the Haematologica Journal website http://www.haematologica.org"Except where otherwise noted, this item's license is described as Atribución-NoComercial 3.0 España. © Ferrata Storti Foundation. Sander B. et al. 2014. The reliability of immunohistochemical analysis of the tumor microenvironment in follicular lymphoma: a validation study from the Lunenburg Lymphoma Biomarker Consortium. "Obtained from the Haematologica Journal website http://www.haematologica.org"