Analysis of ancestral and functionally relevant CD5 variants in systemic lupus erythematosus patients
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AuthorCenit, Maria Carmen; Martínez Florensa, Mario; Consuegra, Marta; Bonet, Lizette; Carnero Montoro, Elena; Armiger, Noelia; Caballero Baños, Miguel; Arias, Maria Teresa; Benitez, Daniel; Ortego Centeno, Norberto; Ramón Garrido, Enrique de; Sabio, José Mario; García Hernández, Francisco J.; Tolosa, Carles; Suárez, Ana; González-Gay Mantecón, Miguel Ángel; Bosch, Elena; Martín, Javier; Lozano, Francisco
Atribución 3.0 España
PLoS One. 2014 Nov 17;9(11):e113090
Public Library of Science
OBJECTIVE: CD5 plays a crucial role in autoimmunity and is a well-established genetic risk factor of developing RA. Recently, evidence of positive selection has been provided for the CD5 Pro224-Val471 haplotype in East Asian populations. The aim of the present work was to further analyze the functional relevance of non-synonymous CD5 polymorphisms conforming the ancestral and the newly derived haplotypes (Pro224-Ala471 and Pro224-Val471, respectively) as well as to investigate the potential role of CD5 on the development of SLE and/or SLE nephritis. METHODS: The CD5 SNPs rs2241002 (C/T; Pro224Leu) and rs2229177 (C/T; Ala471Val) were genotyped using TaqMan allelic discrimination assays in a total of 1,324 controls and 681 SLE patients of Spanish origin. In vitro analysis of CD3-mediated T cell proliferative and cytokine response profiles of healthy volunteers homozygous for the above mentioned CD5 haplotypes were also analyzed. RESULTS: T-cell proliferation and cytokine release were significantly increased showing a bias towards to a Th2 profile after CD3 cross-linking of peripheral mononuclear cells from healthy individuals homozygous for the ancestral Pro224-Ala471 (CC) haplotype, compared to the more recently derived Pro224-Val471 (CT). The same allelic combination was statistically associated with Lupus nephritis. CONCLUSION: The ancestral Ala471 CD5 allele confers lymphocyte hyper-responsiveness to TCR/CD3 cross-linking and is associated with nephritis in SLE patients.