Intracellular ATP production by CD4 T cells: comparison of different conditions of cells sources and clinical utility in renal transplantation

Abstract

The ImmuKnow® uses complete fresh blood to measure the intracellular ATP production. The technique cannot be doing in samples older than 30 hours after the extraction, as manufacturer instructions indicate. Complete blood samples are too labile. The techniques doing in complete blood samples must be processed quickly before they decay. This sometimes complicates the laboratory work when the number of samples to analyse is high. This work is based in the research of kinds of storage that permit analyse the samples at any time without lose its predictive capacity.