Desarrollo de un nanosistema autopropulsado biomimético para aplicaciones en neuronas motoras

Abstract

Neurodegenerative diseases represent a significant public health challenge that will intensify in the coming decades. Their complexity and the lack of effective treatments make them a top priority for biomedical research. The nervous system's complexity and the blood brain barrier hinder the development of traditional drugs, limiting therapeutic options. This study is focused on the development and in vitro investigation of a nanosystem designed to navigate in an ATP environment and target neuronal receptors. This "nanoswimmer" is composed of a fluorescent magnetic nanoparticle core that is functionalized with two genetically engineered proteins. The first is the molecular chaperone HSP90-6XHIS, an ATPase that can refold proteins and prevent amyloid formation. The second protein is the heavy chain of tetanus toxin C, known for its strong affinity for motor neurons, which is used as a targeting mechanism. This project has explored the ability of these “nanoswimmers” to interact with and internalize neuronal cells in culture. This nanosystem offers exciting new possibilities for delivering therapeutic agents to motor neurons to treat neurodegenerative diseases.