New isights into mucosa-associated microbiota in paired tumor and non-tumor adjacent mucosal tissues in colorectal cancer patients

Abstract

Colorectal cancer (CRC) is a major burden of disease worldwide. Increasing scientific evidence highlights the role of the gut microbiota in the initiation, development and treatment of CRC. Currently, the analysis of CRC-associated gut microbiota has several limitations that hinder its implementation in precision medicine, including selection of sample type, sequencing platform and taxonomic classification. This article aims to address these constraints to provide data on CRC-associated microbiota and facilitate the implementation of its analysis in personalized medicine. To this end, mucosa-associated microbiota from paired tumor and non-tumor adjacent tissue samples from 65 CRC patients was analyzed through V3-V4 region of 16S rRNA gene amplification, MinION sequencing and NCBI taxonomic classification. Results consistent with available evidence have been obtained. Moreover, to our knowledge, this is the first study that identifies the possible association between a higher relative abundance of Streptococcus periodonticum and a lower relative abundance of Corynebacterium with CRC.