| dc.contributor.author | Cabezón Navarro, María Elena | |
| dc.contributor.author | Arechaga Iturregui, Ignacio María | |
| dc.contributor.other | Universidad de Cantabria | es_ES |
| dc.date.accessioned | 2024-12-13T15:09:55Z | |
| dc.date.available | 2024-12-13T15:09:55Z | |
| dc.date.issued | 2020 | |
| dc.identifier.issn | 1064-3745 | |
| dc.identifier.issn | 1940-6029 | |
| dc.identifier.other | BFU2016-78521-R | es_ES |
| dc.identifier.other | BFU2014-61823-EXP | es_ES |
| dc.identifier.uri | https://hdl.handle.net/10902/34659 | |
| dc.description.abstract | Biogenesis of T4SS apparatus and substrate transport require energy. Conjugative T4SS have three ATPases that enable DNA processing and transport of the nucleoprotein complex to the recipient cell. In the conjugative plasmid R388, these ATPases are named TrwB, TrwK, and TrwD. Here, three different spectrophotometric assays to measure the enzymatic properties of these ATPases are described. The choice of the assay will depend on the specific requirements of each enzyme. | es_ES |
| dc.description.sponsorship | Acknowledgments: This work was supported by the Spanish Ministerio de Economía y Competitividad (MINECO) grants BFU2016-78521-R (to E.C. and I.A.) and BFU2014-61823-EXP (to E.C.). | es_ES |
| dc.format.extent | 19 p. | es_ES |
| dc.language.iso | eng | es_ES |
| dc.publisher | Springer Nature | es_ES |
| dc.rights | © Springer Science+Business Media, LLC, part of Springer Nature 2020. This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature's AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/978-1-4939-9877-7_10. | es_ES |
| dc.source | Methods in Molecular Biology, 2020, 2075, 135-143 | es_ES |
| dc.subject.other | ATPase activity | es_ES |
| dc.subject.other | Coupled assay | es_ES |
| dc.subject.other | Molecular motor | es_ES |
| dc.subject.other | Spectrophotometric assay | es_ES |
| dc.title | Spectrophotometric assays to quantify the activity of T4SS ATPases | es_ES |
| dc.type | info:eu-repo/semantics/article | es_ES |
| dc.relation.publisherVersion | https://www.doi.org/10.1007/978-1-4939-9877-7_10 | es_ES |
| dc.rights.accessRights | openAccess | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/AEI//BFU2016-78521-R/ES/APLICACIONES BIOMEDICAS Y BIOTECNOLOGICAS DE MOTORES MOLECULARES IMPLICADOS EN LA TRANSFERENCIA DE ADN Y PROTEINAS A TRAVES DE MEMBRANAS BIOLOGICAS/ | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BFU2014-61823-EXP/ES/APLICACION DE UN MOTOR QUE TRANSLOCA SSDNA EN PLATAFORMAS DE SECUENCIACION DE TERCERA GENERACION/ | es_ES |
| dc.identifier.DOI | 10.1007/978-1-4939-9877-7_10 | |
| dc.type.version | acceptedVersion | es_ES |
