Spectrophotometric assays to quantify the activity of T4SS ATPases

Cabezón Navarro, María Elena; Arechaga Iturregui, Ignacio María

doi:10.1007/978-1-4939-9877-7_10

Ver/Abrir

SpectrophotometricAs ... (158.6Kb)

Identificadores

URI: https://hdl.handle.net/10902/34659

DOI: 10.1007/978-1-4939-9877-7_10

ISSN: 1064-3745

ISSN: 1940-6029

Fecha

2020

Derechos

© Springer Science+Business Media, LLC, part of Springer Nature 2020. This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature's AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/978-1-4939-9877-7_10.

Publicado en

Methods in Molecular Biology, 2020, 2075, 135-143

Editorial

Springer Nature

Enlace a la publicación

https://www.doi.org/10.1007/978-1-4939-9877-7_10

Palabras clave

ATPase activity

Coupled assay

Molecular motor

Spectrophotometric assay

Resumen/Abstract

Biogenesis of T4SS apparatus and substrate transport require energy. Conjugative T4SS have three ATPases that enable DNA processing and transport of the nucleoprotein complex to the recipient cell. In the conjugative plasmid R388, these ATPases are named TrwB, TrwK, and TrwD. Here, three different spectrophotometric assays to measure the enzymatic properties of these ATPases are described. The choice of the assay will depend on the specific requirements of each enzyme.