Surface enhanced raman scattering and gated materials for sensing applications: the ultrasensitive detection of mycoplasma and cocaine

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URI: https://hdl.handle.net/10902/31415
ISSN: 0947-6539
ISSN: 1521-3765
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Autoría
Oroval, Mar; Coronado-Puchau, Marc; Langer, Judith; Sanz Ortiz, Marta NorahAutoridad Unican; Ribes, Ángela; Aznar, Elena; Coll, Carmen; Marcos, María Dolores; Sancenón, Félix; Liz-Marzán, Luis M.; Martínez-Máñez, Ramón
Fecha
2016
Derechos
© Wiley-VCH Verlag. This is the peer reviewed version of the following article: Oroval M., Coronado-Puchau M., Langer J., Sanz-Ortiz M.N., Ribes Á., Aznar E., Coll C., Marcos M.D., Sancenón F., Liz-Marzán L.M., Martínez-Máñez R. Surface Enhanced Raman Scattering and Gated Materials for Sensing Applications: The Ultrasensitive Detection of Mycoplasma and Cocaine (2016) Chemistry - A European Journal, 22(38), 13488-13495, which has been published in final form at https://doi.org/10.1002/chem.201602457. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
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Chemistry - A European Journal, 2016, 22(38), 13488-13495
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Wiley-VCH Verlag
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Resumen/Abstract
We present herein a novel combination of gated mesoporous silica nanoparticles (MSNs) and surface-enhanced Raman scattering (SERS) for sensing applications. As a proof-of-concept, we show the design of a system comprising MSNs loaded with crystal violet (CV), a molecule with high Raman cross section acting as SERS reporter, and capped with either a suitable DNA sequence for the detection of Mycoplasma genomic DNA or with an aptamer that selectively coordinates cocaine. In both cases the presence of the corresponding target analyte in solution (i.e., genomic DNA or cocaine) resulted in the release of CV. CV delivery was detected by SERS upon adsorption on gold nanotriangles (AuNTs), which display an efficient electromagnetic field enhancement and a high colloidal stability. By using this novel procedure a limit of detection of at least 30 copies DNA per μL was determined for the detection of Mycoplasma genomic DNA, whereas cocaine was detected at concentrations as low as 10 nM.
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