One-pot synthesis of compact DNA silica particles for gene delivery and extraordinary DNA preservation

Ramos Valle, Andrés; Marín Caba, Laura; García Hevia, Lorena; Correa-Duarte, M. A.; López Fanarraga, Mónica

doi:10.1016/j.mtadv.2023.100357

dc.contributor.author	Ramos Valle, Andrés	es_ES
dc.contributor.author	Marín Caba, Laura	es_ES
dc.contributor.author	García Hevia, Lorena	es_ES
dc.contributor.author	Correa-Duarte, M. A.	es_ES
dc.contributor.author	López Fanarraga, Mónica	es_ES
dc.contributor.other	Universidad de Cantabria	es_ES
dc.date.accessioned	2023-03-27T16:13:10Z
dc.date.available	2023-03-27T16:13:10Z
dc.date.issued	2023	es_ES
dc.identifier.issn	2590-0498	es_ES
dc.identifier.uri	https://hdl.handle.net/10902/28376
dc.description.abstract	Repairing genetic defects using exogenous DNA is a major challenge the science is currently facing. This requires the design of vectors that can effectively encapsulate, protect and target nucleic acids to specific cells safely and precisely. Here we have designed silica-based physiologically responsive particles to encapsulate, store, and transfer DNA. Unlike existing vectors (e.g., viral or lipidic particles), these DNA@SiO2 systems are very stable at room temperature. We also demonstrate how they protect the encapsulated DNA from exposure to different biological and physicochemical stresses, including DNase, denaturation temperatures (>100 °C), or reactive oxygen species (ROS). Remarkably, upon cellular uptake, these vectors dissolve safely unpacking the DNA and transfecting the cells.	es_ES
dc.description.sponsorship	Acknowledgments: We are grateful to D. Munoz for her technical support and Dr. Kirst for his criticisms of the manuscript. MLF acknowledges the financial support from the Spanish Instituto de Salud Carlos iii, and the European Union FEDER funds under Projects ref. PI22/00030 and PI19/00349, co-funded by the European Regional Development Fund, “Investing in your future” and Grant TED2021-129248 BeI00 funded by MCIN/AEI/10.13039/501100011033 and by the “European Union Next Generation EU/PRTR”. We also thank the Gobierno Regional de Cantabria and IDIVAL for the project Refs IDI 20/22, INNVAL21/19, and IDI-020-022 fellowship to ARV and technological and administrative services. MACD acknowledges financial support from the Spanish Ministerio de Economía y Competitividad under grant PID2020-113704RB-I00, Xunta de Galicia/FEDER (IN607A 2018/5 and Centro Singular de Investigacion de Galicia, Acc. 2019e2022, ED431G 2019e06), 0712/ACUINANO1E, 0624/ 2IQBIONEURO6E cofounded by FEDER through the program Interreg V-A Espana-Portugal (POCTEP), and NANOCULTURE (ERDF: 1.102.531) Interreg Atlantic Area, the European Union (European Regional Development Fund-ERDF). LMC acknowledges the Margarita Salas requalification grants for the training of young doctors Ref.: REC-Salas21, Uvigo-UC (Ministerio de Universidades). Figures and graphs have been created with BioRender software (BioRender.com, License ID: 9519A1C8-0002). We are grateful to Dr. Lansford, Dr. Parton, Dr. Greene, Dr. Davidson, Dr. Bement and Dr. Voeltz for the plasmids references in Table S1 (obtained fron Addgene).	es_ES
dc.format.extent	12 p.	es_ES
dc.language.iso	eng	es_ES
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 International	*
dc.rights	© 2023 The Authors	es_ES
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.source	Materials Today Advances 18 (2023) 100357	es_ES
dc.subject.other	Transfection	es_ES
dc.subject.other	Silica	es_ES
dc.subject.other	Gene transfer	es_ES
dc.subject.other	Nanoparticle	es_ES
dc.subject.other	Sequential-gene expresion	es_ES
dc.title	One-pot synthesis of compact DNA silica particles for gene delivery and extraordinary DNA preservation	es_ES
dc.type	info:eu-repo/semantics/article	es_ES
dc.relation.publisherVersion	https://doi.org/10.1016/j.mtadv.2023.100357	es_ES
dc.identifier.DOI	10.1016/j.mtadv.2023.100357	es_ES
dc.type.version	publishedVersion	es_ES