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dc.contributor.authorComins-Boo, Alejandra
dc.contributor.authorRoa-Bautista, Adriel
dc.contributor.authorRenuncio García, Mónica
dc.contributor.authorIrure Ventura, Juan
dc.contributor.authorFariñas Álvarez, María del Carmen 
dc.contributor.authorSan Segundo Arribas, David
dc.contributor.authorLópez Hoyos, Marcos 
dc.contributor.authorGutierrez Larrañaga, María
dc.contributor.authorGuiral Foz, Sandra Almudena
dc.contributor.authorGonzález López, Elena
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2022-04-01T15:38:38Z
dc.date.available2022-04-01T15:38:38Z
dc.date.issued2021-03-23
dc.identifier.issn2296-858X
dc.identifier.urihttp://hdl.handle.net/10902/24487
dc.description.abstractObjectives: Several parameters aid in deciphering between viral and bacterial infections; however, new tools should be investigated in order to reduce the time to results and proceed with an early target-therapy. Validation of a biomarker study, including CD64 and CD169 expression, was conducted. Material and Methods: Patients with active SARS-CoV-2 infection (ACov-2), bacterial infection (ABI), healthy controls, and antiretroviral-controlled chronic HIV infection were assessed. Whole blood was stained and, after lysing no-wash protocol, acquired by flow cytometry. The median fluorescence intensity (MFI) of CD64 and CD169 was measured in granulocytes, monocytes, and lymphocytes. The CD64 MFI ratio granulocytes to lymphocytes (CD64N) and CD169 MFI ratio monocytes to lymphocytes (CD169Mo) were evaluated as biomarkers of acute bacterial and viral infection, respectively. Results: A CD64N ratio higher than 3.3 identified patients with ABI with 83.3 and 85.9% sensitivity and specificity, with an area under the curve (AUC) of 83.5%. In contrast, other analytic or hematological parameters used in the clinic had lower AUC compared with the CD64N ratio. Moreover, a CD169Mo ratio higher than 3.3 was able to identify ACov-2 with 91.7 and 89.8 sensitivity and specificity, with the highest AUC (92.0%). Conclusion: This work confirms the previous data of CD64N and CD169Mo ratios in an independent cohort, including controlled chronic viral HIV infection patients as biomarkers of acute bacterial and viral infections, respectively. Such an approach would benefit from quick pathogen identification for a direct-therapy with a clear application in different Health Care Units, especially during this COVID pandemic.es_ES
dc.description.sponsorshipThis work was partially supported by Instituto de Salud Carlos III Grant: FIS (COV20/0170)es_ES
dc.format.extent7 p.es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Media S.A.es_ES
dc.rights© The authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.es_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceFrontiers in Medicine Volumen 8 Article 655785es_ES
dc.subject.otherBiomarkerses_ES
dc.subject.otherFlow cytometryes_ES
dc.subject.otherValidationes_ES
dc.subject.otherCD169es_ES
dc.subject.otherCD64es_ES
dc.subject.otherSARS-CoV-2es_ES
dc.titleValidation of a Quick Flow Cytometry-Based Assay for Acute Infection Based on CD64 and CD169 Expression. New Tools for Early Diagnosis in COVID-19 Pandemices_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherVersionhttps://doi.org/10.3389/fmed.2021.655785es_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOIdoi.org/10.3389/fmed.2021.655785
dc.type.versionpublishedVersiones_ES


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© The authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Excepto si se señala otra cosa, la licencia del ítem se describe como © The authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.