Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

Pampín Suárez, Sandra; Arce Diego, José Luis; Tapia Martínez, Olga; Pérez Campo, Flor María; Rodríguez Rey, José Carlos; Fanjul Vélez, Félix

doi:10.1364/BOE.447773

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Identificadores

URI: http://hdl.handle.net/10902/23755

DOI: 10.1364/BOE.447773

ISSN: 2156-7085

Fecha

2022-01-01

Derechos

© 2021 Optica Publishing Group under the terms of the Open Access Publishing Agreement. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.

Publicado en

Biomedical Optics Express, 2022, 13(1), 197-208

Editorial

The Optical Society

Enlace a la publicación

https://doi.org/10.1364/BOE.447773

Resumen/Abstract

Biological tissue discrimination is relevant in guided surgery. Nerve identification is critical to avoid potentially severe collateral damage. Fluorescence imaging by oxazine 4-perchlorate (O4P) has been recently proposed. In this work, the cytotoxicity of O4P on U87 human-derived glioma cells has been investigated as a function of concentration and operating room irradiation modes. A custom-built optical irradiation device was employed for controlled optical dosimetry. DNA damage and O4P intracellular localization was also investigated by immunofluorescence and confocal microscopy. The results show that concentration below 100 µM can be considered safe. These results contribute to the assessment of the feasibility of O4P as a nerve biomarker.