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CryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural imaging

dc.contributor.authorPhillips, Michael A.
dc.contributor.authorHarkiolaki, Maria
dc.contributor.authorSusano Pinto, David Miguel
dc.contributor.authorParton, Richard M.
dc.contributor.authorPalanca Cuñado, Ana Rosa 
dc.contributor.authorGarcía Moreno, Manuel
dc.contributor.authorKounatidis, Ilias
dc.contributor.authorSedat, John W.
dc.contributor.authorStuart, David I.
dc.contributor.authorCastello, Alfredo
dc.contributor.authorBooth, Martin J.
dc.contributor.authorDavis, Ilan
dc.contributor.authorDobbie, Ian M.
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2021-01-05T14:22:58Z
dc.date.available2021-01-05T14:22:58Z
dc.date.issued2020
dc.identifier.issn2334-2536
dc.identifier.urihttp://hdl.handle.net/10902/20273
dc.description.abstractRapid cryopreservation of biological specimens is the gold standard for visualizing cellular structures in their true structural context. However, current commercial cryo-fluorescence microscopes are limited to low resolutions. To fill this gap, we have developed cryoSIM, a microscope for 3D super-resolution fluorescence cryo-imaging for correlation with cryo-electron microscopy or cryo-soft X-ray tomography. We provide the full instructions for replicating the instrument mostly from off-the-shelf components and accessible, user-friendly, open-source Python control software. Therefore, cryoSIM democratizes the ability to detect molecules using super-resolution fluorescence imaging of cryopreserved specimens for correlation with their cellular ultrastructure.es_ES
dc.description.sponsorshipFunding: Wellcome Trust (091911/Z/11/Z, 096144/Z/11/Z, 105605/Z/14/Z, 107457/Z/15/Z, 203141/Z/16/Z, 209412/Z/17/Z); H2020Marie Skłodowska-Curie Actions (700184).es_ES
dc.format.extent11 p.es_ES
dc.language.isoenges_ES
dc.publisherOptical Society of Americaes_ES
dc.rightsPublished by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceOptica. 2020 (7): 802-812es_ES
dc.titleCryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural imaginges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherVersionhttps://doi.org/10.1364/OPTICA.393203es_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.1364/OPTICA.393203
dc.type.versionpublishedVersiones_ES


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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.Excepto si se señala otra cosa, la licencia del ítem se describe como Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.