CryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural imaging

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URI: http://hdl.handle.net/10902/20273
ISSN: 2334-2536
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Autoría
Phillips, Michael A.; Harkiolaki, Maria; Susano Pinto, David Miguel; Parton, Richard M.; Palanca Cuñado, Ana RosaAutoridad Unican; García Moreno, Manuel; Kounatidis, Ilias; Sedat, John W.; Stuart, David I.; Castello, Alfredo; Booth, Martin J.; Davis, Ilan; Dobbie, Ian M.
Fecha
2020
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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
Publicado en
Optica. 2020 (7): 802-812
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Optical Society of America
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Resumen/Abstract
Rapid cryopreservation of biological specimens is the gold standard for visualizing cellular structures in their true structural context. However, current commercial cryo-fluorescence microscopes are limited to low resolutions. To fill this gap, we have developed cryoSIM, a microscope for 3D super-resolution fluorescence cryo-imaging for correlation with cryo-electron microscopy or cryo-soft X-ray tomography. We provide the full instructions for replicating the instrument mostly from off-the-shelf components and accessible, user-friendly, open-source Python control software. Therefore, cryoSIM democratizes the ability to detect molecules using super-resolution fluorescence imaging of cryopreserved specimens for correlation with their cellular ultrastructure.
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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.Excepto si se señala otra cosa, la licencia del ítem se describe como Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.