Mostrar el registro sencillo

dc.contributor.authorGiner Robles, Laura
dc.contributor.authorLázaro Pinto, Beatriz
dc.contributor.authorCruz Calahorra, Fernando de la 
dc.contributor.authorMoncalián Montes, Gabriel 
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2019-03-18T18:51:56Z
dc.date.available2019-03-18T18:51:56Z
dc.date.issued2018
dc.identifier.issn1475-2859
dc.identifier.otherBFU2014-55534-C2
dc.identifier.urihttp://hdl.handle.net/10902/15912
dc.description.abstractBackground: Some marine bacteria, such as Moritella marina, produce the nutraceutical docosahexaenoic acid (DHA) thanks to a specific enzymatic complex called Pfa synthase. Escherichia coli heterologously expressing the pfa gene cluster from M. marina also produces DHA. The aim of this study was to find genetic or metabolic conditions to increase DHA production in E. coli. Results: First, we analysed the effect of the antibiotic cerulenin, showing that DHA production increased twofold. Then, we tested a series of single gene knockout mutations affecting fatty acid biosynthesis, in order to optimize the synthesis of DHA. The most effective mutant, fabH, showed a threefold increase compared to wild type strain. The combination of cerulenin inhibition and fabH deletion rendered a 6.5-fold improvement compared to control strain. Both strategies seem to have the same mechanism of action, in which fatty acid synthesis via the canonical pathway (fab pathway) is affected in its first catalytic step, which allows the substrates to be used by the heterologous pathway to synthesize DHA. Conclusions: DHA-producing E. coli strain that carries a fabH gene deletion boosts DHA production by tuning down the competing canonical biosynthesis pathway. Our approach can be used for optimization of DHA production in different organisms.es_ES
dc.description.sponsorshipFunding: The work in the FdlC and GM laboratories was financed by the Spanish Ministry of Economy, Industry and Competitiveness Grant BFU2014-55534-C2.es_ES
dc.format.extent9 p.es_ES
dc.language.isoenges_ES
dc.publisherBioMed Centrales_ES
dc.rightsAttribution 4.0 Internationales_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceMicrob Cell Fact. 2018 Jun 8;17(1):88.es_ES
dc.subject.otherFatty Acid Synthesises_ES
dc.subject.otherPolyunsaturated Fatty Acides_ES
dc.subject.otherDocosahexaenoic Acides_ES
dc.subject.otherCerulenines_ES
dc.subject.otherFabHes_ES
dc.titlefabH deletion increases DHA productionin Escherichia coli expressing Pfa geneses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherVersionhttps://doi.org/10.1186/s12934-018-0928-5es_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.1186/s12934-018-0928-5
dc.type.versionpublishedVersiones_ES


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo

Attribution 4.0 InternationalExcepto si se señala otra cosa, la licencia del ítem se describe como Attribution 4.0 International