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dc.contributor.authorPérez Campo, Flor María es_ES
dc.contributor.authorMay, Tobiases_ES
dc.contributor.authorZauers, Jeannettees_ES
dc.contributor.authorSañudo Campo, María Carolina es_ES
dc.contributor.authorDelgado Calle, Jesúses_ES
dc.contributor.authorArozamena García, Jana es_ES
dc.contributor.authorBerciano Blanco, María Teresa es_ES
dc.contributor.authorLafarga Coscojuela, Miguel Ángel es_ES
dc.contributor.authorRiancho Moral, José Antonio es_ES
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2018-02-14T19:17:31Z
dc.date.available2018-03-01T03:45:12Z
dc.date.issued2017-03es_ES
dc.identifier.issn0914-8779es_ES
dc.identifier.issn1435-5604es_ES
dc.identifier.urihttp://hdl.handle.net/10902/13052
dc.description.abstractDifferent model systems using osteoblastic cell lines have been developed to help understand the process of bone formation. Here, we report the establishment of two human osteoblastic cell lines obtained from primary cultures upon transduction of immortalizing genes. The resulting cell lines had no major differences to their parental lines in their gene expression profiles. Similar to primary osteoblastic cells, osteocalcin transcription increased following 1,25-dihydroxyvitamin D3 treatment and the immortalized cells formed a mineralized matrix, as detected by Alizarin Red staining. Moreover, these human cell lines responded by upregulating ALPL gene expression after treatment with the demethylating agent 5-aza-2 Œ-deoxycytidine (AzadC), as shown before for primary osteoblasts. We further demonstrate that these cell lines can differentiate in vivo, using a hydroxyapatite/tricalcium phosphate composite as a scaffold, to produce bone matrix. More importantly, we show that these cells respond to demethylating treatment, as shown by the increase in SOST mRNA levels, the gene encoding sclerostin, upon treatment of the recipient mice with AzadC. This also confirms, in vivo, the role of DNA methylation in the regulation of SOST expression previously shown in vitro. Altogether our results show that these immortalized cell lines constitute a particularly useful model system to obtain further insight into bone homeostasis, and particularly into the epigenetic mechanisms regulating sclerostin production.es_ES
dc.format.extent11 p.es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.rights© Springer. The final publication is available at Springer via http://dx.doi.org/10.1007/s00774-016-0753-zes_ES
dc.sourceJ Bone Miner Metab 35 (2), 150-160es_ES
dc.titleGeneration and characterization of two immortalized human osteoblastic cell lines useful for epigenetic studieses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOIDOI 10.1007/s00774-016-0753-zes_ES
dc.type.versionacceptedVersiones_ES


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