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Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers

dc.contributor.authorSantos, Carolina S.es_ES
dc.contributor.authorRamos, Juliana N.es_ES
dc.contributor.authorVieira, Veronica V.es_ES
dc.contributor.authorPinheiro, Carina S.es_ES
dc.contributor.authorMeyer, Robertoes_ES
dc.contributor.authorAlcántara Neves, Neuza M.es_ES
dc.contributor.authorRamos, Rommel T.es_ES
dc.contributor.authorSilva, Artures_ES
dc.contributor.authorHirata, Raphaeles_ES
dc.contributor.authorFelicori, Lizaes_ES
dc.contributor.authorRuiz de Alegría Puig, Carloses_ES
dc.contributor.authorNavas Méndez, Jesús es_ES
dc.contributor.authorAzevedo, Vascoes_ES
dc.contributor.authorMattos Guaraldi, Ana L.es_ES
dc.contributor.authorPacheco, Luis G.C.es_ES
dc.contributor.otherUniversidad de Cantabriaes_ES
dc.date.accessioned2017-10-16T17:44:20Z
dc.date.available2018-11-01T03:45:10Z
dc.date.issued2017-11es_ES
dc.identifier.issn0167-7012es_ES
dc.identifier.issn1872-8359es_ES
dc.identifier.urihttp://hdl.handle.net/10902/12105
dc.description.abstractA multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226 bp, 434 bp and 106 bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.es_ES
dc.description.sponsorshipThis work was supported by grants from FAPESB (JCB0031/2013) and CAPES (PROCAD 071/2013).es_ES
dc.format.extent13 p.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rights© <2017> Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 licensees_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceJournal of Microbiological Methods Volume 142, November 2017, Pages 33-35es_ES
dc.subject.otherEmerging pathogenses_ES
dc.subject.otherCorynebacterium sppes_ES
dc.subject.otherBacterial identificationes_ES
dc.subject.otherMultiplex PCRes_ES
dc.subject.otherVitek-MSes_ES
dc.titleEfficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primerses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherVersionhttps://doi.org/10.1016/j.mimet.2017.09.002es_ES
dc.rights.accessRightsopenAccesses_ES
dc.identifier.DOI10.1016/j.mimet.2017.09.002es_ES
dc.type.versionacceptedVersiones_ES


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© <2017> Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 licenseExcepto si se señala otra cosa, la licencia del ítem se describe como © <2017> Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license