Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers

Santos, Carolina S.; Ramos, Juliana N.; Vieira, Veronica V.; Pinheiro, Carina S.; Meyer, Roberto; Alcántara Neves, Neuza M.; Ramos, Rommel T.; Silva, Artur; Hirata, Raphael; Felicori, Liza; Ruiz de Alegría Puig, Carlos; Navas Méndez, Jesús; Azevedo, Vasco; Mattos 
Guaraldi, Ana L.; Pacheco, Luis G.C.

doi:10.1016/j.mimet.2017.09.002

Ver/Abrir

EfficientDifferentia ... (640.6Kb)

Identificadores

URI: http://hdl.handle.net/10902/12105

DOI: 10.1016/j.mimet.2017.09.002

ISSN: 0167-7012

ISSN: 1872-8359

Fecha

2017-11

Derechos

Publicado en

Journal of Microbiological Methods Volume 142, November 2017, Pages 33-35

Editorial

Elsevier

Enlace a la publicación

https://doi.org/10.1016/j.mimet.2017.09.002

Palabras clave

Emerging pathogens

Corynebacterium spp

Bacterial identification

Multiplex PCR

Vitek-MS

Resumen/Abstract

A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226 bp, 434 bp and 106 bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.

Colecciones a las que pertenece

D02 Artículos [403]
IDIVAL Artículos [864]

© <2017> Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license

Excepto si se señala otra cosa, la licencia del ítem se describe como © <2017> Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license