dc.contributor.author | Cenit, Maria Carmen | |
dc.contributor.author | Martínez Florensa, Mario | |
dc.contributor.author | Consuegra, Marta | |
dc.contributor.author | Bonet, Lizette | |
dc.contributor.author | Carnero Montoro, Elena | |
dc.contributor.author | Armiger, Noelia | |
dc.contributor.author | Caballero Baños, Miguel | |
dc.contributor.author | Arias, Maria Teresa | |
dc.contributor.author | Benitez, Daniel | |
dc.contributor.author | Ortego Centeno, Norberto | |
dc.contributor.author | Ramón Garrido, Enrique de | |
dc.contributor.author | Sabio, José Mario | |
dc.contributor.author | García Hernández, Francisco J. | |
dc.contributor.author | Tolosa, Carles | |
dc.contributor.author | Suárez, Ana | |
dc.contributor.author | González-Gay Mantecón, Miguel Ángel | |
dc.contributor.author | Bosch, Elena | |
dc.contributor.author | Martín, Javier | |
dc.contributor.author | Lozano, Francisco | |
dc.contributor.other | Universidad de Cantabria | es_ES |
dc.date.accessioned | 2017-01-26T17:43:11Z | |
dc.date.available | 2017-01-26T17:43:11Z | |
dc.date.issued | 2014-11 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10902/10171 | |
dc.description.abstract | OBJECTIVE: CD5 plays a crucial role in autoimmunity and is a well-established genetic risk factor of developing RA. Recently, evidence of positive selection has been provided for the CD5 Pro224-Val471 haplotype in East Asian populations. The aim of the present work was to further analyze the functional relevance of non-synonymous CD5 polymorphisms conforming the ancestral and the newly derived haplotypes (Pro224-Ala471 and Pro224-Val471, respectively) as well as to investigate the potential role of CD5 on the development of SLE and/or SLE nephritis.
METHODS: The CD5 SNPs rs2241002 (C/T; Pro224Leu) and rs2229177 (C/T; Ala471Val) were genotyped using TaqMan allelic discrimination assays in a total of 1,324 controls and 681 SLE patients of Spanish origin. In vitro analysis of CD3-mediated T cell proliferative and cytokine response profiles of healthy volunteers homozygous for the above mentioned CD5 haplotypes were also analyzed.
RESULTS: T-cell proliferation and cytokine release were significantly increased showing a bias towards to a Th2 profile after CD3 cross-linking of peripheral mononuclear cells from healthy individuals homozygous for the ancestral Pro224-Ala471 (CC) haplotype, compared to the more recently derived Pro224-Val471 (CT). The same allelic combination was statistically associated with Lupus nephritis.
CONCLUSION: The ancestral Ala471 CD5 allele confers lymphocyte hyper-responsiveness to TCR/CD3 cross-linking and is associated with nephritis in SLE patients. | es_ES |
dc.format.extent | 9 p. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.rights | Atribución 3.0 España | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | PLoS One. 2014 Nov 17;9(11):e113090 | es_ES |
dc.title | Analysis of ancestral and functionally relevant CD5 variants in systemic lupus erythematosus patients | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | openAccess | es_ES |
dc.identifier.DOI | 10.1371/journal.pone.0113090 | |
dc.type.version | publishedVersion | es_ES |