@article{10902/38504,
year = {2025},
url = {https://hdl.handle.net/10902/38504},
abstract = {Objectives Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is associated with significant alterations in gut microbiota (GM) composition, affecting transplant success. This study aimed to correlate these GM changes with post-transplant (post-HSCT) outcomes. Methods A prospective multicentre cohort study was conducted between June 2017 and December 2021 in three Spanish hospitals. Stool samples from allo-HSCT recipients were collected before HSCT, and at 14-, 30-, 60-, and 100-days post-HSCT. Bacterial 16S rRNA gene sequences were characterized and microbial diversity assessed. Results Analysis of 409 samples from 95 patients revealed significant longitudinal GM shifts. Alpha diversity significantly decreased at days 14 ( P < 0.001), 30 ( P < 0.001), and 60 ( P = 0.002) compared to baseline. A distinct shift in dominant taxonomic profiles was observed, notably a significant decrease in Blautia abundance ( P < 0.001). Patients with acute gastrointestinal graft-versus-host disease (GI-GVHD) ( P = 0.009), bacteraemia ( P = 0.014), or death ( P < 0.001) exhibited significantly lower Blautia levels. LEfSe analysis identified 22 differential taxa between deceased and surviving patients; the former showed higher abundance of potential pathogens such as Enterococcus_H ( P = 0.026), Enterococcus_A ( P = 0.019), and Staphylococcus ( P = 0.009). Conclusions Significant variations in the GM?s taxonomic profiles and relative abundances post-HSCT, particularly the decrease in Blautia and the increase in certain pathogens, are associated with poorer clinical outcomes.},
organization = {This work was supported by Instituto de Salud Carlos III
through the project ‘PI16/01415’ (Co-funded by European Regional
Development Fund ‘A way to make Europe’) under Grant number [PI16/01415 to MCF]; Instituto de Investigación Marqués de
Valdecilla (IDIVAL) through the emerging researchers support program, ‘NEXT-VAL’ 2021 under Grant number [NVAL21/18 to CGR];
and CIBER -Consorcio Centro de Investigación Biomédica en Red-(CB21/13/00068), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea—NextGenerationEU.},
publisher = {Elsevier},
publisher = {International Journal of Infectious Diseases, 2025, 161, 108117},
title = {Changes in the bacterial profile and diversity of the gut microbiota in allogeneic hematopoietic stem cell transplant recipients},
author = {González Rico, Claudia and Hernández, Marta and Rodríguez Grande, Jorge and Fernández Luis, Sara and Bermúdez Rodríguez, María Aranzazu and González Huerta, Ana Julia and Llaneza Velasco, Eugenia and Vázquez López, Lourdes and García García, Inmaculada and Arnaiz de las Revillas Almajano, Francisco and Fariñas Álvarez, María Concepción and Calvo Montes, Jorge and Ocampo Sosa, Alain and Fernández Martínez, Marta and Fariñas Álvarez, María del Carmen},
}