@mastersthesis{10902/38036,
year = {2025},
month = {9},
url = {https://hdl.handle.net/10902/38036},
abstract = {The RAS-ERK signalling pathway is essential for a multitude of cellular events, including cell proliferation and survival. Its dysregulation is closely associated with the tumourigenic progression of several types of human cancer, such as melanoma. In recent years, lncRNA have emerged as a key regulator of signalling, mainly modulating gene transcription, although new cytoplasmic functions have been characterised. Recently, FP236383.2 (FP) has been described as a possible modulator of MEK kinase activity, however, the mechanisms of interaction between them remain poorly characterised.
The aim of this study is to analyse the binding between FP and MEK under conditions of RAS-ERK pathway stimulation, as well as to identify the region of MEK involved in its interaction with FP. To this end, HEK293T cells transfected for MEK overexpression were used. Protein-RNA interactions were evaluated by CLIP assays, followed by RT-PCR and qPCR for the detection of FP in MEK immunocomplexes.
The results showed that stimulation with EGF enhanced FP binding to MEK, indicating that pathway activation strengthens this interaction. Among the MEK fragments analysed, the region corresponding to amino acids 262-393 showed the highest affinity for FP, suggesting that it constitutes the main binding domain.
Overall, this study identifies a specific region of MEK a responsible for FP binding and demonstrates that this binding is modulated by extracellular stimuli. Considering the central role of MEK in RAS oncogenic signalling, the FP-MEK axis emerges as a potential therapeutic target for the development of new strategies against the aberrant activation of the RAS-ERK pathway.},
title = {Caracterización de la Interacción del lncRNA FP236383.2 con la Proteína MEK en la Vía de Señalización Celular RAS-ERK},
author = {Goya López, Verna},
}