@article{10902/37431,
year = {2025},
url = {https://hdl.handle.net/10902/37431},
abstract = {Background: The nuclear basket is a "fishtrap"-like structure on the nucleoplasmic face of the nuclear pore complex which has been implicated in diverse functions including RNA export, heterochromatin organisation, and mitosis. Recently, a novel component of the nuclear basket, ZC3HC1, has been described. The localisation of ZC3HC1 to nuclear pores has been reported to occur reciprocally with TPR, a major structural component of the nuclear basket.
Methods: Using siRNA-mediated knock down, immunofluorescence and RNA sequencing we compare the consequences of depleting two proteins of the nuclear pore basket - TPR and ZC3HC1.
Results: We show that in human fibroblasts, although ZC3HC1 localisation to nuclear pores is TPR-dependent, TPR localises to pores regardless of the presence of ZC3HC1. We demonstrate that knockdown of TPR and ZC3HC1 produce distinct transcriptional profiles.
Conclusions: Our results suggest that there is little overlap in function between these two nuclear basket proteins in human diploid fibroblasts.},
organization = {This work was supported by Wellcome [217120; Wellcome Trust Investigator Award to W.A.B]. B.B was supported a
PhD studentship from the Medical Research Council. Work in the WAB lab is funded by MRC University Unit grant MC_UU_00035/7. J.C.A.
acknowledges funding by Cancer Research UK (CRUK) (C47559/A16243 Training & Career Development Board - Career Development
Fellowship), the University of Edinburgh-MRC Chancellor’s Fellowship, the Ministry of Science and Innovation of the Government of Spain
(Proyecto PID2020-117860GB-I00 financed by MCIN/ AEI /10.13039/ 501100011033) and the Spanish National Research Council (CSIC).
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.},
publisher = {Taylor & Francis},
publisher = {Wellcome Open Research, 2025, 10, 188},
title = {ZC3HC1 has functions distinct from TPR and is dispensable for TPR localisation to the nuclear basket},
author = {Bartlett, Bethany M. and Acosta Cobacho, Juan Carlos and Bickmore, Wendy A.},
}