@article{10902/36123,
year = {2022},
url = {https://hdl.handle.net/10902/36123},
abstract = {Bacteria are powerful models for understanding how cells divide and accomplish global regulatory programs. In Caulobacter crescentus, a cascade of essential master regulators supervises the correct and sequential activation of DNA replication, cell division, and development of different cell types. Among them, the response regulator CtrA plays a crucial role coordinating all those functions. Here, for the first time, we describe the role of a novel factor named CcnA (cell cycle noncoding RNA A), a cell cycle-regulated noncoding RNA (ncRNA) located at the origin of replication, presumably activated by CtrA, and responsible for the accumulation of CtrA itself. In addition, CcnA may be also involved in the inhibition of translation of the S-phase regulator, GcrA, by interacting with its 50 untranslated region (50 UTR). Performing in vitro experiments and mutagenesis, we propose a mechanism of action of CcnA based on liberation (ctrA) or sequestration (gcrA) of their ribosome-binding site (RBS). Finally, its role may be conserved in other alphaproteobacterial species, such as Sinorhizobium meliloti, representing indeed a potentially conserved process modulating cell cycle in Caulobacterales and Rhizobiales.},
organization = {Funding: This research was funded by the Agence Nationale Recherche (ANR; ANR-17-CE20-0011- 01) to EGB. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgments: We thank members of the Biondi and Massé’s laboratory for critical comments on the manuscript. We thank the IMM Transcriptomic facility for the RNA preparation and the qRT-PCR experiment; we also thank Artemis Kosta and Hugo le Guenno from the IMM Microscopy platform for Electron Microscopy acquisition and analysis. We thank also Gaël Panis and Patrick Viollier for the phage CbK and also for providing the delta cpdR, rcdA, popA strains used in this work. We thank Regis Hallez and Romain Mercier for MreB and GFP antibodies, respectively, used in this study.},
publisher = {Public Library os Science},
publisher = {PLoS biology, 2022, 20(2), e3001528},
title = {The noncoding RNA CcnA modulates the master cell cycle regulators CtrA and GcrA in Caulobacter crescentus},
author = {Beroual, Wanassa and Prévost, Karine and Close
Lalaouna, David and Zaina, Nadia Ben and Valette, Odile and Denis, Yann and Djendli, Meriem and Brasseur, Gaël and Brilli, Matteo and Robledo Garrido, Marta and Jimenez Zurdo, Jose Ignacio and Massé, Eric},
}