@mastersthesis{10902/35387,
year = {2024},
month = {6},
url = {https://hdl.handle.net/10902/35387},
abstract = {Squamous Cell Carcinoma (SCC) is a frequent solid tumour and a major cause of mortality. In
non-epidermoid epithelia SCC may arise from squamous metaplasia (SQM). Even though it is
known to arise from environmental hazard, the mechanisms leading to SQM are still unknown.
Previous work in the host lab has shown that DNA damage in the non-squamous cells of the
human lung or the mammary gland, leads to squamous metaplasia. This might explain why
SCC is frequent in the lung (30% of the tumours) but very rare in the mammary gland, less
exposed to genotoxics. We aimed to study this precancerous process in a physiological
mimicking 3D system. We wanted to study the relationship between the DNA damage
epithelium and the SMQ, by using Mammary Gland Organoids (MGO). In this TFM, I have
focused on the development of MGOs from mouse, first and human second, mammary gland.
To this end, I have set up a method to analyze mammary branching following the induction of
DNA damage or the block of mitosis. I have then studied the capacity of the MGOs to undergo
SQM in response to severe DNA damage or to replication stress. These MGOs will constitute
a good model to study the molecular and cellular mechanisms driving SQM.},
title = {The making of mammary organoids to study mechanisms of squamous metaplasia},
author = {Augeri, Pietro},
}