@article{10902/34515,
year = {2024},
url = {https://hdl.handle.net/10902/34515},
abstract = {Cellular senescence is a cell fate triggered in response to stress and is characterized by stable cell-cycle arrest and a hypersecretory state. It has diverse biological roles, ranging from tissue repair to chronic disease. The development of new tools to study senescence in vivo has paved the way for uncovering its physiological and pathological roles and testing senescent cells as a therapeutic target. However, the lack of specific and broadly applicable markers makes it difficult to identify and characterize senescent cells in tissues and living organisms. To address this, we provide practical guidelines called "minimum information for cellular senescence experimentation in vivo" (MICSE). It presents an overview of senescence markers in rodent tissues, transgenic models, non-mammalian systems, human tissues, and tumors and their use in the identification and specification of senescent cells. These guidelines provide a uniform, state-of-the-art, and accessible toolset to improve our understanding of cellular senescence in vivo.},
organization = {Acknowledgments: we thank Eirini Klinaki, who developed original illustrations for Figures 2 and 3. We thank the Society of the Advancement of Research in Shock/Sepsis and Tissue Regeneration and Trauma Care Consult for supporting the “Wiggers-Bernard Conference: Senescence in vivo” and the MICSE workshop in Vienna. The Research Group Senescence and Healing of Wounds (H.R., M.O., and N.A.R.R.) is a collaboration between the Ludwig Boltzmann Gesellschaft and the Austrian Workers’ Compensation Board, with support from the Austrian Nationalstiftung. M.O. is supported by Austrian Science Fund (Der Österreichischer Wissenschaftsfonds; FWF) grant DOIs https://doi.org/10.55776/P35382, https://doi.org/10.55776/P36483, and https://doi.org/10.55776/P37321 and a Federation of European Biochemical Societies Excellence (FEBS) Award. N.A.R.R. is supported by Austrian Science Fund (Der Österreichischer Wissenschaftsfonds; FWF) grant DOI: https://doi.org/10.55776/P36483 and a Federation of European Biochemical Societies Excellence (FEBS) Award. P.A. is supported by NIH research grants R01 AG071861, U54 AG079758, and P01 AG031862. Core support from MRC (MC_U120085810) and a grant from CRUK (C15075/A28647) funded research in J. Gil’s laboratory. The research of K.S.-K. is funded by the German Research Foundation within the Collaborative Research Center CRC1506 (project ID 450627322) “Aging at Interfaces” and the Graduate Training Group GRK 1789 “Cellular and Molecular Mechanisms in Ageing (CEMMA).” Work in the laboratory of M.C. is funded by grant PID2021-125479OB-I00 from MCINN/AEI/FEDER, UE. J. Grillari kindly acknowledges support by the Austrian Science Fund (Der Österreichischer Wissenschaftsfonds; FWF) and ‘Herzfelder’sche Familienstiftung project P35268-B. F.d.d.F’s laboratory is supported by ERC advanced grant (TELORNAGING—835103); AIRC-IG (21762); Telethon (GGP17111); AIRC 5X1000 (21091); Progetti di Ricerca di Interesse Nazionale (PRIN) 2015 “ATR and ATM-mediated control of chromosome integrity and cell plasticity”; Progetti di Ricerca di Interesse Nazionale (PRIN) 2017 “RNA and genome Instability”; Progetto AriSLA 2021 “DDR & ALS”; POR FESR 2014-2020 Regione Lombardia (InterSLA project); FRRB—Fondazione Regionale per la Ricerca Biomedica—under the frame of EJP RD, the European Joint Programme on Rare Diseases with funding from the European Union’s Horizon 2020 research and innovation program under the EJP RD COFUND-EJP NO 825575; co-funding European Union – Next Generation EU, in the context of The National Recovery and Resilience Plan, Investment Partenariato Esteso PE8 “Conseguenze e sfide dell'invecchiamento,” Project Age-It (Ageing Well in an Ageing Society), and National Center for Gene Therapy and Drugs based on RNA Technology, CN00000041. C.L.B. is funded by the Biotechnology and Biological Sciences Research Council (BBSRC, grant BB/X006972/1) L.J.N. and P.D.R. are supported by P01 AG043376, U19 AG056278, RO1 AG063543, P01 AG062413, U54 AG079754, and U54 AG076041 from the National Institutes of Health and an Aligning Science Across Parkinson’s grant ASAP-000592 from the Michael J. Fox Foundation. F.R is supported by grants from the Canadian Institute for Health Research (PJT173341), the Natural Sciences and Engineering Research Council (NSERC – RGPIN-2022-04385) by a FRQS junior I-II-senior career awards (22624, 33070, and 281706), and the Cancer Research Society (CRS) in partnership with Ovarian Cancer Canada (20087). T.v.Z. is supported by BBSRC grant no. BB/S006710/1 and H2020 WIDESPREAD Project 857524. D.J.B. is supported by grants from the National Institutes of Health (R01AG053229, R01AG068076, and U54AG079779) and the Glenn Foundation for Medical Research. E.S. was supported by the National Science Center, grant UMO-2019/35/B/NZ4/01920. F.G. gratefully acknowledge the financial support of the Federal Ministry for Labour and Economy of Austria and the National Foundation for Research, Technology, and Development of Austria to the Christian Doppler Laboratory for Skin Multimodal Imaging of Aging and Senescence. Work in the Jansen-Dürr lab was supported by the Austrian Science Funds (FWF Project FG 2400 B, SENIOPROM) and the European Union (MSCA Project COFUND-ARDRE). J.F.P. would like to acknowledge funding from the NIH grants R01AG068048, R01AG82708, UG3CA268103, and The Glenn Foundation For Medical Research.
D.J. would like to acknowledge funding from NIH grant R01 AG068182 and Hevolution/AFAR.
M.H.Y. is supported by DFG (22137416, 450807335, and 497658823) grants and TUD & CRTD funds. J.L.K. is supported by NIH grants R37AG013925 and R33AG061456; The Connor Fund, Robert J. and Theresa W. Ryan, and the Noaber Foundation. V.G. is supported by the National Public Investment Program of the Ministry of Development and Investment/General Secretariat for Research and Technology, in the framework of the Flagship Initiative to address SARS-CoV-2 (2020ΣΕ01300001); the European Regional Development Fund of the European Union and Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation, under the call RESEARCH–CREATE–INNOVATE (project codes: T2EDK-02939 and T2EDK-03266); Sonia Kotopoulos donation; P. Galanis and Co. donation (lab supplies); and NKUA-SARG grant 70/3/8916. V.K. is supported by European Research Council grant 856487, Israel Science Foundation grants 2633/17 and 1626/20, and DFG-CRC 1506 “Aging at Interfaces.” M.D. is funded by the Dutch Research Council (NWO) Talent Program (VIDI 09150172010029), Dutch Organization for Health Research and Development (ZonMw), Dutch Cancer Foundation (KWF), Health Holland, American Federation for Aging Research (AFAR), and Hevolution Foundation. We are grateful to Barbara Bachmann, Teresa Brandtner, Helene Dworak, Ines Fischer, Eirini Klinaki, Razieh Khoshnevisan, Javier Orihuel, Tomaz Rozmaric, Cornelia Schneider, Paul Slezak, Tanja Szikora, Karla Valdivieso, and Susanne Windwarder for their help in organizing the “Wiggers-Bernard Conference: Senescence in vivo” and the MICSE workshop.},
publisher = {Elsevier (Cell Press)},
publisher = {Cell, 2024, 187, 4150-4175},
title = {Guidelines for minimal information on cellular senescence experimentation in vivo},
author = {Ogrodnik, Mikolaj and Acosta Cobacho, Juan Carlos and Adams, Peter D. and D'Adda di Fagagna, Fabrizio and Baker, Darren J. and Bishop, Cleo  L. and Chandra, Tamir and Collado, Manuel and Gil, Jesús and Gorgoulis, Vassilis and Gruber, Florial and Hara, Eiji and Jansen-Dürr, Pidder and Jurk, Diana and Khosla, Sundeep and Kirkland, James L. and Krizhanovsky, Valery and Minamino, Tohru and Niedernhofer, Laura J. and Passos, Joao F.},
}