@article{10902/33810,
year = {2024},
url = {https://hdl.handle.net/10902/33810},
abstract = {Intracellular pathogens like Brucella face challenges during the intraphagocytic adaptation phase, where the modulation of gene expression plays an essential role in taking advantage of stressors to persist inside the host cell. This study aims to explore the expression of antisense virB2 RNA strand and related genes under intracellular simulation media. Sense and antisense virB2 RNA strands increased expression when nutrient deprivation and acidification were higher, being starvation more determinative. Meanwhile, bspB, one of the T4SS effector genes, exhibited the highest expression during the exposition to pH 4.5 and nutrient abundance. Based on RNA-seq analysis and RACE data, we constructed a regional map depicting the 5' and 3' ends of virB2 and the cis-encoded asRNA_0067. Without affecting the CDS or a possible autonomous RBS, we generate the deletion mutant ΔasRNA_0067, significantly reducing virB2 mRNA expression and survival rate. These results suggest that the antisense asRNA_0067 expression is promoted under exposure to the intraphagocytic adaptation phase stressors, and its deletion is associated with a lower transcription of the virB2 gene. Our findings illuminate the significance of these RNA strands in modulating the survival strategy of Brucella within the host and emphasize the role of nutrient deprivation in gene expression.},
organization = {Funding: This study was funded by DGAPA-PAPIIT (UNAM), grant No. IN218421 Adrian Muñoz-Bucio received a scholarship from CONACYT, Mexico.
Acknowledgements: We appreciate the insights from Juan Miranda Ríos into the world of regulatory RNAs and the technical assistance Omar Cortéz Hernández and Adolfo Ortiz Rico provided.},
publisher = {Springer Nature},
publisher = {Archives of Microbiology, 2024, 206, 285},
title = {Increased Brucella abortus asRNA_0067 expression under intraphagocytic stressors is associated with enhanced virB2 transcription},
author = {Muñoz-Bucio, Adrián and Arellano-Reynoso, Beatriz and Sangari García, Félix Javier and Sieira, Rodrigo and Thébault, Patricia and Espitia, Clara and García Lobo, Juan María and Seoane Seoane, Asunción and Suárez-Güemes, Francisco},
}