@article{10902/32208,
year = {2012},
url = {https://hdl.handle.net/10902/32208},
abstract = {Although new therapies have doubled the survival of multiple myeloma patients, this remains an incurable disease. It has been postulated that the so-called myeloma cancer stem cells would be responsible for tumor initiation and relapse but their unequivocal identification remains unclear. Here, we investigated in a panel of myeloma cell lines the presence of CD20+ cells harboring a stem-cell phenotype. Thus, only a small population of CD20dim+ cells (0.3%) in the RPMI-8226 cell line was found. CD20dim+ RPMI-8226 cells expressed the plasma cell markers CD38 and CD138 and were CD19?CD27?. Additionally, CD20dim+ RPMI-8226 cells did not exhibit stem-cell markers as shown by gene expression profiling and the aldehyde dehydrogenase assay. Furthermore, we demonstrated that CD20dim+ RPMI-8226 cells are not essential for CB17-SCID mice engraftment and show lower self-renewal potential than the CD20? RPMI-8226 cells. These results do not support CD20 expression for the identification of myeloma cancer stem cells.},
publisher = {Ferrata Storti Foundation},
publisher = {Haematologica, 2012, 97(7), 1110-1114},
title = {CD20 positive cells are undetectable in the majority of multiple myeloma cell lines and are not associated with a cancer stem cell phenotype.},
author = {Paíno, Teresa and Ocio San Miguel, Enrique María and Paiva, Bruno and San-Segundo, Laura and Garayoa, Mercedes and Gutiérrez, Norma C. and Eugenia Sarasquete M. and Pandiella, Atanasio and Orfao, Alberto and San Miguel, Jesús F.},
}