@article{10902/31493,
year = {2016},
url = {https://hdl.handle.net/10902/31493},
abstract = {Most bacteria in nature exist as biofilms, which support intercellular signalling processes such as quorum sensing (QS), a cell-to-cell communication mechanism that allows bacteria to monitor and respond to cell density and changes in the environment. As QS and biofilms are involved in the ability of bacteria to cause disease, there is a need for the development of methods for the non-invasive analysis of QS in natural bacterial populations. Here, by using surface-enhanced resonance Raman scattering spectroscopy, we report rationally designed nanostructured plasmonic substrates for the in situ, label-free detection of a QS signalling metabolite in growing Pseudomonas aeruginosa biofilms and microcolonies. The in situ, non-invasive plasmonic imaging of QS in biofilms provides a powerful analytical approach for studying intercellular communication on the basis of secreted molecules as signals.},
organization = {This work has been funded by the European Research Council (ERC Advanced Grant no. 267867 Plasmaquo). E.H.H. gratefully acknowledges the Spanish Ministry of Economy and Competitiveness for funding a Juan de la Cierva Fellowship. V.M.-G. acknowledges an FPU scholarship from the Spanish MINECO.},
publisher = {Nature Publishing Group},
publisher = {Nature Materials, 2016, 15, 1203-1211},
title = {Detection and imaging of quorum sensing in Pseudomonas aeruginosa biofilm communities by surface-enhanced resonance Raman scattering},
author = {Bodelón, Gustavo and Montes-García, Verónica and López-Puente, Vanesa and Hill, Eric H. and Hamon, Cyrille and Sanz Ortiz, Marta Norah and Rodal-Cedeira, Sergio and Costas, Celina and Celiksoy, Sirin and Pérez-Juste, Ignacio and Scarabelli, Leonardo and La Porta, Andrea and Pérez-Juste, Jorge and Pastoriza-Santos, Isabel and Liz-Marzán, Luis M.},
}