@article{10902/30902,
year = {2018},
url = {https://hdl.handle.net/10902/30902},
abstract = {Online organ monitoring could provide clinicians with critical information regarding organ health prior to
transplantation and could aid clinical decision-making. This paper presents the methodology of online
microdialysis for real-time monitoring of human organs ex vivo. We describe how rapid sampling
microdialysis can be incorporated with organ perfusion machines to create a robust organ monitoring
system and demonstrate its use in monitoring human and porcine kidneys as well as human and porcine
pancreases. In this paper we also show the potential usefulness of this methodology for evaluating novel
interventions in a research setting. The analysis system can be configured either to analyse two analytes
in one organ, allowing for ratiometric analysis, or alternatively to monitor one analyte in two organs
simultaneously, allowing direct comparison. It was found to be reliable over long monitoring periods in
real clinical use. The results clearly show that the analysis system is sensitive to differences between
organs and therefore has huge potential as an ex vivo organ monitoring tool.},
organization = {The research was supported by the National Institute for Health Research Imperial Biomedical Research Centre based at Impe rial College Healthcare NHS Trust and Imperial College London, the Imperial Experimental Cancer Medicine Centre and the Cancer Research UK Imperial Centre at Imperial College
London. The views expressed are those of the authors and not necessarily those of the NHS, the NIHR or the Department of
Health. We would also like to thank the Wellcome Trust DOH (HICF-0510-080)},
publisher = {Royal Society of Chemistry},
publisher = {Analytical Methods, 2018, 10(44), 5245-5350},
title = {An improved rapid sampling microdialysis system for human and porcine organ monitoring in a hospital setting},
author = {Gowers, Sally A. N. and Hamaoui, Karim and Vallant, Natalie and Hanna, George B. and Darzi, Ara and Casanova Rituerto, Daniel and Papalois, Vassilios and Boutelle, Martyn G.},
}