@misc{10902/30524,
year = {2023},
month = {6},
url = {https://hdl.handle.net/10902/30524},
abstract = {The rough endoplasmic reticulum (ER) is an organelle which contributes to cell 
homeostasis. When that cellular equilibrium is broken, ER acts as a stress sensor and 
activates the misfolded protein pathway (UPR), key to control cell fate decisions.
Seipin is a transmembrane protein of the RER encoded by BSCL2 gene and its 
localization is ubiquitous. It is a multifunctional molecule, whose possible functions in 
the organism are still being hypothesized. However, it is known that it plays a 
fundamental role in lipid metabolism. 
At the cellular level, seipin is essential in the adipocyte differentiation and, at the 
tissue level, it is considered a tissue-dependent protein highly relevant in the gonads, 
liver and nervous tissue. Therefore, the synthesis of an aberrant, mutated seipin 
and/or its aggregation will lead to pathologies mainly related to previously mentioned 
tissues and all of them are called seipinopathies.
Due to the fact that the disorders included in this group are immeasurable, this Final 
Degree Project will focus into reviewing lipodystrophy type 2 and neurodegenerative 
diseases; the latter mentioned, from a more generalized point of view, due to their 
lack of knowledge because of their recent appearance in the literature.},
abstract = {In recent years there has been a need to use and develop new human models of 
preclinical experimentation that more accurately reflect the biological 
characteristics of human beings. Currently, three-dimensional human models 
have been developed that, together with the incorporation of biomedical 
engineering techniques and artificial intelligence, have represented an important 
advance in the field of preclinical research. 
This literature review will aim to present an updated summary of certain human 
models of interest available for preclinical research. The evolution from 2D cell 
cultures to human three-dimensional (3D) models will be analyzed to address the 
most promising preclinical models such as spheroids, organoids and assoids 
derived from human cells. In addition, the main characteristics, limitations and 
applications of the same will be exposed. 
Finally, examples of intestinal and brain organoids will be described in more 
detail, as well as some diseases that have benefited from the study with 
organoids in recent years},
title = {Experimentación preclínica en modelos humanos},
author = {Domínguez Zotes, Inés},
}