@article{10902/29657,
year = {2023},
url = {https://hdl.handle.net/10902/29657},
abstract = {GES (Guiana Extended Spectrum) carbapenemases belong to “minor class A carbapenemases” and its
prevalence could be underestimated due to the lack of specific tests. The aim of this study was to
develop an easy PCR method to differentiate between GES β-lactamases with or without
carbapenemase activity, based on an allelic discrimination system of SNPs that encode E104K and G170S
mutations, without need of sequencing.
Two pair of primers and Affinity Plus probes, labeled with different fluorophores; FAM/IBFQ and
YAK/IBFQ, were designed for each one of the SNPs.
This allelic discrimination assay allows to detect in real time the presence of all type of GES- βlactamases, being able to differentiate between carbapenemases and extended-spectrum β-lactamase
(ESBL), through a quick PCR test that avoid costly sequencing approaches and could help to decrease the
current underdiagnosis of minor carbapenemases that scape of phenotypic screenings.},
organization = {Funding: This research was supported by grant from the Instituto de Salud Carlos III (number PI18CIII/00030) CIBER – Consorcio Centro de Investigación Biomédica en Red (CB21/13/00068 and CB21/13/00095) (CIBERINFEC) and Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea – NextGenerationEU also supported this work.},
publisher = {Elsevier},
publisher = {Journal of Microbiological Methods, 2023, 207, 106694},
title = {Are GES carbapenemases underdiagnosed? An allelic discrimination assay for their accurate detection and differentiation},
author = {Pablo-Marcos, D. and Siller, M. and Agüero Balbín, Jesús and Álvarez-Justel, A. and García-Fernández, S. and Velasco de la Fuente, S. and Goicoechea, P. and Rodríguez-Lozano, J. and Ocampo-Sosa, A. and Lucas-Fernández, J. and Fariñas Álvarez, María del Carmen and Fernández, J. and Freile-Ribot, P.A. and Aracil, B. and Oteo-Iglesias, J. and Calvo-Montes, J.},
}