@article{10902/28843,
year = {2022},
url = {https://hdl.handle.net/10902/28843},
abstract = {Eukaryotic cells divide and separate all their components after chromosome segregation by a process called cytokinesis to complete cell division. Cytokinesis is highly regulated by the recruitment of the components to the division site and through post-translational modifcations such as phosphorylations. The budding yeast mitotic kinases Cdc28-Clb2, Cdc5, and Dbf2- Mob1 phosphorylate several cytokinetic proteins contributing to the regulation of cytokinesis. The PP2A-Cdc55 phosphatase regulates mitosis counteracting Cdk1- and Cdc5-dependent phosphorylation. This prompted us to propose that PP2A-Cdc55 could also be counteracting the mitotic kinases during cytokinesis. Here we show that in the absence of Cdc55, AMR contraction and the primary septum formation occur asymmetrically to one side of the bud neck supporting a role for PP2A-Cdc55 in cytokinesis regulation. In addition, by in vivo and in vitro assays, we show that PP2A-Cdc55 dephosphorylates the chitin synthase II (Chs2 in budding yeast) a component of the Ingression Progression Complexes (IPCs) involved in cytokinesis. Interestingly, the non-phosphorylable version of Chs2 rescues the asymmetric AMR contraction and the defective septa formation observed in cdc55? mutant cells. Therefore, timely dephosphorylation of the Chs2 by PP2A-Cdc55 is crucial for proper actomyosin ring contraction. These fndings reveal a new mechanism of cytokinesis regulation by the PP2A-Cdc55 phosphatase and extend our knowledge of the involvement of multiple phosphatases during cytokinesis.},
organization = {Funding: Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. We thank CERCA Program/Generalitat de Catalunya for institutional support. EQ is funded by the grants BFU2016-77975-R (co-funded by the European Regional Development Fund, ERDF, a way to build Europe) from the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) and PID2019-109027GB-I00 from the Spanish Ministry of Science, Innovation and Universities (MCIU). ASD was supported by the grant PID2019 106745GB-I00 from the Spanish Spanish Ministry of Science, Innovation and Universities (co-funded by the European Regional Development Fund) and a grant from the Consejería de Universidades, Investigación, Medio Ambiente y Política Social del Gobierno de Cantabria.},
publisher = {Springer},
publisher = {Cellular and Molecular Life Sciences (2022) 79: 165},
title = {PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis},
author = {Moyano-Rodríguez, Yolanda and Vaquero, David and Vilalta-Castany, Odena and Foltman, Magdalena and Sánchez Díaz, Alberto and Queralt, Ethel},
}